FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18635658
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC10741015.7636870133590135TruSeq Adapter, Index 6 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC7185573.855817701741468No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT3960172.1250497299317255No Hit
GTGGACGTGCCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC2974391.5960745791750417TruSeq Adapter, Index 5 (97% over 35bp)
GTGGACGTGCCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC2322471.2462506019374255TruSeq Adapter, Index 6 (100% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT2200401.1807471461431627No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC2114601.134706378492243No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC1570740.8428680114219739No Hit
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA1143510.6136139652273078TruSeq Adapter, Index 6 (100% over 38bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA1042900.5596260674026107TruSeq Adapter, Index 6 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC1000960.5371208250333849No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT995940.5344270645018276Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC989150.5307835119103388No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT921390.494423110791151Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA812900.4362067601798659No Hit
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA583120.3129055062075082TruSeq Adapter, Index 6 (100% over 37bp)
GTGGACGTGCCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG554270.29742443223630743Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC538800.2891231423113689No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA459040.24632347298925533Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT447140.23993786535468722Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCGGGACGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA425650.22840620921461427TruSeq Adapter, Index 6 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT369970.1985280047530385No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT367190.19703624095269404No Hit
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG361300.1938756334764246Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA345250.18526311225501133Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGACGTGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA342300.18368012548845872TruSeq Adapter, Index 6 (100% over 37bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT335270.17990778753291137Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG302610.16238224590728162No Hit
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC258730.13883598851191625TruSeq Adapter, Index 6 (100% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCT254350.13648565561784834TruSeq Adapter, Index 6 (100% over 51bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC249290.13377043085894794No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC249240.13374360057476908No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTCT218290.11713565466805627Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA199670.10714405683984972TruSeq Adapter, Index 6 (100% over 37bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA194600.10442346602411356TruSeq Adapter, Index 6 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)