FastQCFastQC Report
Wed 17 Jul 2024
EGAF00001839267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19369088
Total Bases987.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG10893455.624141931721308TruSeq Adapter, Index 7 (100% over 49bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG4097012.1152312385590895No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3659431.889314561429015No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT3206801.6556277714263057No Hit
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1292430.6672642511614382TruSeq Adapter, Index 7 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1217360.6285066183807931Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT837960.43262749387064586No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG803530.4148517472789633No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC736200.38009017254710187No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG723220.3733887728735602TruSeq Adapter, Index 7 (100% over 36bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC645140.33307711751838803Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC578300.2985685232056357No Hit
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG535270.27635271211530454TruSeq Adapter, Index 7 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG390000.20135176214801648No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT386840.19972029658804794No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT335230.1730747467304604No Hit
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG322100.16629590407147718TruSeq Adapter, Index 7 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT286910.14812777968689078No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC269610.13919602203263262TruSeq Adapter, Index 7 (100% over 50bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG260970.13473530607119963TruSeq Adapter, Index 7 (100% over 36bp)
GCATTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA253570.13091478545608343Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC217340.11220972303910229Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC209680.10825496791588743No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)