FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16402317
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC16144299.84268868843347TruSeq Adapter, Index 12 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC10939396.669417497540134No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT5161593.1468663847918563No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG966140.5890265381409224TruSeq Adapter, Index 12 (100% over 37bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT772740.47111636727908623TruSeq Adapter, Index 12 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT725710.44244358891490754No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC702840.42850043685901207Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG610060.37193525768341146TruSeq Adapter, Index 12 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC603160.3677285349380822No Hit
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA452760.27603417248916723TruSeq Adapter, Index 12 (100% over 40bp)
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT403860.24622131129400804TruSeq Adapter, Index 12 (100% over 41bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC370930.22614487940941516Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT351870.21452456991289703TruSeq Adapter, Index 12 (100% over 38bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT335360.20445891882226153TruSeq Adapter, Index 12 (100% over 38bp)
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA322270.19647833900539782TruSeq Adapter, Index 12 (100% over 40bp)
AAGGCCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA312970.19080840834864976TruSeq Adapter, Index 12 (100% over 40bp)
ACGGCCCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA275090.1677141101467555TruSeq Adapter, Index 12 (100% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCT274810.16754340255708994TruSeq Adapter, Index 12 (100% over 51bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG271410.1654705246825799TruSeq Adapter, Index 12 (100% over 37bp)
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA261550.15945917884650077TruSeq Adapter, Index 12 (100% over 39bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC260910.1590689900701224Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG260050.15854467390186397TruSeq Adapter, Index 12 (100% over 49bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG215070.13112171896202224Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT211120.12871352260781208TruSeq Adapter, Index 12 (100% over 36bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT207010.12620777905950725TruSeq Adapter, Index 12 (100% over 38bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA200600.12229979459609273Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA198730.12115971176511221TruSeq Adapter, Index 12 (100% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT197520.12042201110977187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG195910.11944044246919505TruSeq Adapter, Index 12 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT192030.11707492301240123No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC188520.11493498144195116Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGCCCGGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT175850.10721046300958578TruSeq Adapter, Index 12 (100% over 36bp)
AGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA171060.10429014388637899TruSeq Adapter, Index 12 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)