FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18470638
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT9722115.263548557445606No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC3702502.0045328158128592No Hit
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC2351191.2729338315222245TruSeq Adapter, Index 1 (100% over 36bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC2009781.0880945206115782Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1941991.0513930271385319No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1618000.8759849010088336TruSeq Adapter, Index 1 (100% over 50bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1553590.8411133389111952TruSeq Adapter, Index 1 (100% over 37bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1048420.567614394261855Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC1000770.5418166930671263Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC898450.486420663974899TruSeq Adapter, Index 1 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT847320.4587388914232416No Hit
ACGGCCCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG733100.3969002045300222TruSeq Adapter, Index 1 (100% over 39bp)
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT709120.38391743696130043Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA703930.38110757192036354Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC647010.35029109443864365TruSeq Adapter, Index 1 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACGT600540.32513224502586213No Hit
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA578220.313048201150388Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT548970.2972122565555126Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT498000.26961710797428873No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC446940.24197323340969598Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA429740.23266115658809403Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG413070.2236360216685531Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC397520.2152172545420467No Hit
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA353360.1913090387023989TruSeq Adapter, Index 1 (100% over 37bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA334900.1813147981136331TruSeq Adapter, Index 1 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC325630.17629602182664184No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT321280.17394093263048088No Hit
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC313830.16990750400717072Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT313660.16981546603858513Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC308560.16705432698101713TruSeq Adapter, Index 1 (100% over 36bp)
AAATGGATTTTTGGAAATAGGAGAAGATCGGAAGAGCACACGTCTGAACTC301920.16345943220802658Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC281670.15249608595003594No Hit
GAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG278230.15063367058571556TruSeq Adapter, Index 1 (100% over 39bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA277440.15020596473170011Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC273320.14797539749303734TruSeq Adapter, Index 1 (100% over 38bp)
AAGGCCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA266400.14422891077178818TruSeq Adapter, Index 1 (100% over 40bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA266100.14406649082722536No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG260980.14129452377335316No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG259950.14073688196368744Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC224770.12169043646462023No Hit
AACGGGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG215210.1165146542312182TruSeq Adapter, Index 1 (100% over 39bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA213780.11574045249546874TruSeq Adapter, Index 1 (100% over 37bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA202160.10944938664273535Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA199940.10824747905297046TruSeq Adapter, Index 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)