FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001839277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122476
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT95947.8333714360364475No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC48003.919135177504164TruSeq Adapter, Index 2 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC29872.4388451615010287No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC26082.1293967797772626No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC10140.8279173062477546Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT9080.7413697377445377No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC6350.5184689245239884No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG5510.4498840589176655Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC4500.3674189228910154Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA4360.3559881119566282Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG4220.3445573010222411TruSeq Adapter, Index 2 (100% over 38bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT4210.34374081452692773TruSeq Adapter, Index 2 (100% over 37bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT4160.3396583820503609Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC3860.31516378719095983Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT3790.30944838172376626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA3510.286586759854992Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA3100.25311081354714393Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA2990.2441294620986969Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT2980.24331297560338355No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG2780.22698324569711617Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC2680.2188183807439825No Hit
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG2520.2057545968189686TruSeq Adapter, Index 2 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT2450.20003919135177503No Hit
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT2400.1959567588752082Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA2400.1959567588752082TruSeq Adapter, Index 2 (100% over 36bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC2340.191057839903328Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT2010.16411378555798686TruSeq Adapter, Index 2 (100% over 39bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA1810.1477840556517195TruSeq Adapter, Index 2 (100% over 36bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT1760.14370162317515267TruSeq Adapter, Index 2 (100% over 39bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1630.1330872987360789TruSeq Adapter, Index 2 (100% over 37bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG1550.12655540677357197Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG1480.12084000130637838TruSeq Adapter, Index 2 (100% over 38bp)
AGATCGGAAGAGCACACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1470.12002351481106503Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA1470.12002351481106503TruSeq Adapter, Index 2 (100% over 40bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1460.11920702831575164TruSeq Adapter, Index 2 (100% over 37bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA1420.11594108233449818TruSeq Adapter, Index 2 (100% over 40bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT1400.11430810934387145TruSeq Adapter, Index 2 (100% over 51bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG1390.11349162284855809No Hit
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1350.11022567686730463Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC1280.10451027140011104Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC1260.1028772984094843No Hit
GGGATTGTGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG1260.1028772984094843TruSeq Adapter, Index 2 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC1240.10124432541885758No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)