FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20507167
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT248683612.126667715730797No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC10453565.0975154198529715No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT2308631.1257673963448975No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2221971.0835089995609828TruSeq Adapter, Index 3 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1987430.9691392282512743No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG787550.38403646881112347Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT780160.380432850622419No Hit
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA751590.3665011359199445TruSeq Adapter, Index 3 (100% over 40bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC721080.35162341048863555Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC652490.318176567245978Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG581330.28347650360481286TruSeq Adapter, Index 3 (100% over 37bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC467200.22782278995436084Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC420780.20518680127781666No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC419280.20445534968335705No Hit
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA418580.20411400560594256TruSeq Adapter, Index 3 (100% over 40bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA416420.20306071530992068Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA407390.19865737671127368Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT392260.1912794682951575TruSeq Adapter, Index 3 (100% over 41bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC389370.18987020488983194TruSeq Adapter, Index 3 (100% over 39bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG353770.17251042038132328TruSeq Adapter, Index 3 (100% over 38bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC337270.1644644528422673No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG319280.15569191005271474Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG314950.15358045311670793TruSeq Adapter, Index 3 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG309470.15090821662494874No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT288380.14062400720684629Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA271310.1323000880618956Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG259140.12636557745884647TruSeq Adapter, Index 3 (100% over 38bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT253440.12358606139989985Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC251690.12273270120636362TruSeq Adapter, Index 3 (100% over 39bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT249430.1216306474707111Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTTGGTCCGAGTGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA246560.12023113675331165Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC209950.10237884150453352Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)