FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13734884
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC8927036.499530684059654No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT6670094.856313311419303No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA5737514.1773268707620685Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC2989422.176516379752461Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2077231.5123753502395798Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1749281.273603766875643Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1528271.1126923241579616No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA1340730.9761494891402067Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1296370.9438521650419471TruSeq Adapter, Index 1 (100% over 36bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1163380.8470257193289729TruSeq Adapter, Index 1 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1088640.7926095335060711No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT1010350.7356086880675512Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1006370.7327109570055342Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1006140.7325435001853674TruSeq Adapter, Index 1 (100% over 50bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT968480.7051242660658801No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA937710.6827214558200855TruSeq Adapter, Index 1 (100% over 37bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC893750.6507153609742901Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA842510.6134088937336494Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA781140.5687270456743574Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG739740.5385848180443316Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA632330.4603824830264311Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT629410.45825650948344376Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA613400.44660005865357144TruSeq Adapter, Index 1 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC596790.43450676394500315No Hit
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG498380.3628570871075431Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT486640.35430950854772414Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC482590.351360812366526No Hit
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA477030.3473127257572762TruSeq Adapter, Index 1 (100% over 37bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC435740.3172505861716779TruSeq Adapter, Index 1 (100% over 38bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC426160.3102756455751647TruSeq Adapter, Index 1 (100% over 36bp)
GGGGGCGGGGAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT395810.2881786260444573TruSeq Adapter, Index 1 (100% over 35bp)
GCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT390510.2843198384493091Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC390280.2841523816291423Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACGT352080.25633998801882857No Hit
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC329550.23993650037379277TruSeq Adapter, Index 1 (100% over 38bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAAC296250.21569166510616325Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAGCTGGGTTGAGAGGGCGAAAGATCGGAAGAGCACACGTCTGAACTCC283150.20615390708796666Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGCCCTGGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT281280.20479241033269738TruSeq Adapter, Index 1 (100% over 35bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC275120.20030747984475153TruSeq Adapter, Index 1 (100% over 36bp)
AAAAGCTGGGTTGAGAGGGCGATAGATCGGAAGAGCACACGTCTGAACTCC261160.1901435789337573Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT258380.18811953562913236Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC257960.18781374491404515No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT256010.18639400230828304Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG254510.18530189261154298No Hit
AAAAGCTGGGTTGAGAGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA254360.18519268164186897Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCCCTGGCGGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA248500.1809261730932711Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG238280.17348526569281547No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT238160.17339789691707624No Hit
CGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT231810.16877463253421TruSeq Adapter, Index 1 (100% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT217020.1580064309243529No Hit
AGGGGCCCGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA216550.1576642365527077TruSeq Adapter, Index 1 (100% over 37bp)
GGGACGGCCGGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT203480.14814832072844591TruSeq Adapter, Index 1 (100% over 35bp)
ACGGCCCTGGCGGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC200130.14570927573905976Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA193560.14092583526733826Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT181440.1321015889176785No Hit
AAAAGCTGGGTTGAGAGGGCGAATAGATCGGAAGAGCACACGTCTGAACTC177740.12940771833238635Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT175490.12776955378727625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA171680.12499559515755647TruSeq Adapter, Index 1 (100% over 37bp)
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA163240.1188506579305657TruSeq Adapter, Index 1 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT156690.11408177892146742No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGAGATCGGAAGAGCACACGTCTGA152780.11123501297863163Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC150150.10932018064368071No Hit
TCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA149410.10878140652662228Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAAGAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT148630.10821350948431745TruSeq Adapter, Index 1 (100% over 35bp)
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC146980.10701218881790338TruSeq Adapter, Index 1 (100% over 38bp)
GAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT145990.10629139641805493TruSeq Adapter, Index 1 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)