FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20266955
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT8422814.155932649971345No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC4060142.003330051307658No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2682021.3233463043659002TruSeq Adapter, Index 7 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2071071.0218950010003969No Hit
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC1980990.9774482649218887Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1627760.8031596261007141TruSeq Adapter, Index 7 (100% over 37bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC1218420.6011855258967123Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC715640.35310681846384917Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT713750.35217426594177564TruSeq Adapter, Index 7 (100% over 38bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA659350.32533254255511007Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC643570.3175464691168456TruSeq Adapter, Index 7 (100% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT604830.29843160948450326No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG600630.29635927054656214Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC583390.2878528126203468Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG553890.2732970986514748Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA514580.2539009930204118TruSeq Adapter, Index 7 (100% over 37bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA453990.22400503677044728TruSeq Adapter, Index 7 (100% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG443680.2189179380918347No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT414720.204628667700698Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA412930.20374545658190885Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA410210.20240337041257553TruSeq Adapter, Index 7 (100% over 40bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA332710.16416378286723388Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC328080.16187927589517023No Hit
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT301520.148774199182857Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT286000.14111641339313183Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA279640.13797830014424958TruSeq Adapter, Index 7 (100% over 37bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC274610.1354964275590487Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC264960.13073498214211263TruSeq Adapter, Index 7 (100% over 39bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA255360.1259982074268187TruSeq Adapter, Index 7 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT254220.12543571542937754No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC248420.12257391403888744No Hit
AAATGGATTTTTGGAAATAGGAGAAGATCGGAAGAGCACACGTCTGAACTC225870.11144742759827513Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC217750.107440905651589Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT206240.1017617101335647No Hit
AACGGGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC203370.10034561185930496TruSeq Adapter, Index 7 (100% over 39bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT202930.10012850968485398No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)