FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17908685
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT12709937.0970760834757No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC6016573.359582236216674No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC3575061.9962716413851715TruSeq Adapter, Index 11 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2725411.5218370304687363No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1247480.6965782244760015No Hit
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA783920.4377317485901393TruSeq Adapter, Index 11 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC618330.34526823158707637No Hit
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC584590.32642821066985095TruSeq Adapter, Index 6 (97% over 36bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG571320.3190183980565854Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT542330.3028307215186375No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA495760.27682657883591116Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC466870.2606947411270007Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT429470.23981101906700575TruSeq Adapter, Index 11 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG428870.23947598609278123No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT413800.2310610745568421Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT409760.2288051858637304No Hit
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC382500.21358352106812978TruSeq Adapter, Index 6 (97% over 36bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT367780.2053640454338216TruSeq Adapter, Index 11 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC341730.19081803046957385No Hit
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT332100.18544075123327033TruSeq Adapter, Index 11 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT329950.18424021640896582Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG312670.1745912667512997Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT299080.1670027698851144TruSeq Adapter, Index 11 (100% over 37bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC292490.16332299105154846No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC282220.15758834330940547No Hit
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA271780.15175876955789885TruSeq Adapter, Index 11 (100% over 38bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC269850.15068108015747667Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC253560.14158493490728102No Hit
AGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC243010.13569393844383326TruSeq Adapter, Index 11 (100% over 39bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT238400.13311976842520823TruSeq Adapter, Index 11 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA218340.12191849932030187No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC214540.11979662381687992No Hit
AAGGCCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA213000.11893670584970364TruSeq Adapter, Index 11 (100% over 40bp)
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT212000.11837831755932945TruSeq Adapter, Index 11 (100% over 41bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA209420.11693767577016403Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT205430.11470970649157099No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCT198460.11081774010766283TruSeq Adapter, Index 11 (100% over 51bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT180850.10098452231417326Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)