FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18757372
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC14419477.687361534440966No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT11300816.024729903528064No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT3380131.8020274908446665Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA1609170.8578867018258208Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1372960.731957547144664No Hit
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1323810.7057545161443725TruSeq Adapter, Index 2 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1181990.630146909705688TruSeq Adapter, Index 2 (100% over 50bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC1096500.5845701626006031Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC936340.49918506707656063No Hit
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC685740.36558426201708855Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC610580.32551468297371294Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG576460.3073245015346499Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA551920.2942416453648198TruSeq Adapter, Index 2 (100% over 40bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC540490.28814804120747833No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT509210.2714719311425929TruSeq Adapter, Index 2 (100% over 37bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT509080.27140262505856366TruSeq Adapter, Index 2 (100% over 39bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC509010.27136530639793255Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT471970.2516184036868278No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT463900.24731609523978093Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG445200.23734668161403422No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA433880.23131172106625597Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC400010.21325482055801845Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT382730.20404244261936055Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA374650.1997348029350807TruSeq Adapter, Index 2 (100% over 40bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC324820.17316924780294382Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT320840.171047415384202No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT310290.1654229601033663No Hit
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT294310.1569036430050009TruSeq Adapter, Index 2 (100% over 39bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC263130.1402808453124457No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT250380.13348351784034565Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT248670.1325718762734993Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG237650.12669685284271165TruSeq Adapter, Index 2 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT230670.12297564925406394Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT217100.11574116032885631TruSeq Adapter, Index 2 (100% over 41bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC212640.11336342852292954No Hit
ACGGCCCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT211110.11254774922627754TruSeq Adapter, Index 2 (100% over 39bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC203350.1084107091334543No Hit
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT202080.1077336420048608TruSeq Adapter, Index 2 (100% over 37bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG197280.10517464813301139TruSeq Adapter, Index 2 (100% over 38bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG194560.10372455160563003Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)