FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19737923
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC5277472.673771703334743No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA3717431.883394721926922Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT2979561.5095610617186013Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT2021821.0243327020781263No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1968030.9970805945488794Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1621060.8212920883316852TruSeq Adapter, Index 6 (100% over 50bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA1579210.8000892495122208TruSeq Adapter, Index 6 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT998950.5061069495508722No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC860020.4357196043372953No Hit
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA758880.3844781439262885TruSeq Adapter, Index 6 (100% over 38bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG578090.29288289350404295Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT575100.2913680431319952Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA527970.26749015081272737TruSeq Adapter, Index 6 (100% over 40bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA493290.249919913052655TruSeq Adapter, Index 6 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC467680.2369448903007677No Hit
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC463030.2345890193208272TruSeq Adapter, Index 6 (100% over 36bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG358500.18163005296960577Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC313790.15897822683774784No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT254360.12886867579734707No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC251230.1272828959764409No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT251030.12718156819235743Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT244730.12398974299372836TruSeq Adapter, Index 6 (100% over 39bp)
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA243470.12335137795400256TruSeq Adapter, Index 6 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT242560.1228903365364228No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA237190.12016968553378185Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT236010.11957185160768942Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC224130.11355298123313178No Hit
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT209180.10597872937289299TruSeq Adapter, Index 6 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)