FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001839304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16620171
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT347576420.91292562513346No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC13776778.28918667563649No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT4382832.637054696970326No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC3857462.3209508494226685No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC3842422.311901604381808No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT3344592.0123679834581725No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC2894181.7413659582684198Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT2427961.4608513955722837Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG2219891.3356601445316056No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC1969731.185144244304105No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC1837321.1054759905899885Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA1801441.083887765053681Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT1698921.022203682501221No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1667321.003190641058988Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG1419010.8537878461057951No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG1227790.7387348782392191Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT1027140.6180080818663056No Hit
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC950300.5717751038782934TruSeq Adapter, Index 4 (100% over 37bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT938850.5648858847481172Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC888010.5342965484530815No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT823230.4953198134965037No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA764440.4599471329145771Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT733120.44110256145980686Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG681950.4103146712509757Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT592420.35644639275973755No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC554670.33373302837858887No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACGT548230.3298582186669439No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC537740.32354661092235454TruSeq Adapter, Index 4 (100% over 50bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCCA531450.3197620529897075Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT522320.31426872804136613Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTGGGTTGAGAGGGCGATAGATCGGAAGAGCACACGTCTGAACTCC461130.2774520189954724Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA438660.26393230250158073Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT381880.22976899575822657Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAGCTGGGTTGAGAGGGCGAAAGATCGGAAGAGCACACGTCTGAACTCC378130.227512701283278Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGAATAGATCGGAAGAGCACACGTCTGAACTC368000.2214176978082837Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC349860.21050324933479928No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC335970.20214593459958988No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT331380.19938423016225287Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA322440.1940052241339755Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC317020.19074412651951655TruSeq Adapter, Index 4 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT286820.17257343501459763Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT278250.1674170500411819Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT257620.15500442203633163Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAAC252640.15200806297359998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC245680.14782038042809548No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG241030.1450225752791593Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGGAAGAGCACACG239620.14417420855657861No Hit
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT239220.14393353714591745Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGACTGGTTGACCAGAGGGGAGATCGGAAGAGCACACGTCTGAACTCCA234260.14094921165371885Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC224820.13526936636211506No Hit
TGAGGGGCAGAGAGCGAGACTTTTAGATCGGAAGAGCACACGTCTGAACTC223470.13445710035113356Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT220730.1328085011881045No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA211610.12732119302502964TruSeq Adapter, Index 2 (97% over 36bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTCT210410.1265991787930461Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCAAGATCGGAAGAGCACAC198180.1192406504120806No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGTC196640.11831406548103506No Hit
GCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT194920.11727917841519199Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC192960.11609988850295222No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGN192060.11555837782896458No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG188480.11340436870354703Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG179780.1081697655216664No Hit
GGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT178690.1075139359276147Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGGGCAGAGAGCGAGACTTCAGATCGGAAGAGCACACGTCTGAACTCC177690.10691225740096176Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCCA170320.10247788665952956Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC169910.10223119846360185Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTG168520.10139486531155426Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)