FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001839306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12306410
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC9055927.358701684731778No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT6214565.049856131885741No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG2926192.377777109652612Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2532602.0579519128649215TruSeq Adapter, Index 6 (100% over 50bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT2166801.7607084438109895Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC1636671.329932937387914Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1487131.208419027157392Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC1440611.1706175887200247TruSeq Adapter, Index 6 (100% over 36bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG1259261.0232553604178636Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA1021020.8296651907420605TruSeq Adapter, Index 6 (100% over 37bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC943340.766543614262811Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA938710.76278134728162Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG916930.7450832533614596No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT912340.7413534897667151No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA791440.6431120042319409TruSeq Adapter, Index 6 (100% over 37bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT554270.4503913001435837Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA548600.4457839451147816TruSeq Adapter, Index 6 (100% over 38bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT539880.43869820686942823No Hit
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG514960.41844859711321175Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC505330.4106234068261987No Hit
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC473750.38496198322662745Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG427970.3477618574385219Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA399240.3244163001232691TruSeq Adapter, Index 6 (100% over 37bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT375310.3049711491816054Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA370710.3012332597402492Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC357020.29010897572890876TruSeq Adapter, Index 6 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT356110.28936952368724916No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT354860.2883537928607937Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG354360.2879475005302115No Hit
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT298450.24251589212451072Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAGCTGGGTTGAGAGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA294340.2391761691671251Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC294110.2389892746950573TruSeq Adapter, Index 6 (100% over 36bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC275720.22404584277624426TruSeq Adapter, Index 6 (100% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT272590.2215024527867997No Hit
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA262060.21294593630473874TruSeq Adapter, Index 6 (100% over 37bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA260040.21130451528918667TruSeq Adapter, Index 6 (100% over 38bp)
AGAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA256890.2087448736065189TruSeq Adapter, Index 6 (100% over 37bp)
AGAAGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG255490.20760725508088873Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA254660.2069328098121223Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGCCCGGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC248820.2021873153909223TruSeq Adapter, Index 6 (100% over 36bp)
AGATCGGCAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC247670.20125284303058327TruSeq Adapter, Index 6 (98% over 50bp)
GAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC247450.20107407440512706TruSeq Adapter, Index 5 (97% over 35bp)
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA243800.19810814039187707TruSeq Adapter, Index 6 (100% over 38bp)
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA238750.19400458785299693Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT230850.18758516902979827No Hit
GCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT198210.16106240568939276Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC195910.15919346096871467Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGCACGTGTTAGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC189990.15438295977462152Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT186590.1516201719266626TruSeq Adapter, Index 6 (100% over 39bp)
AGGGGCCCGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA185480.15071820295277014TruSeq Adapter, Index 6 (100% over 37bp)
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGTCTGAACTCCAG182260.14810168034382082Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC181350.14736222830216125Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAAC178740.14524138233652217Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC170230.13832628687001328No Hit
ACGGCCCTGGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC168710.1370911581850434TruSeq Adapter, Index 5 (97% over 35bp)
AAAAGCTGGGTTGAGAGGGCGAAAGATCGGAAGAGCACACGTCTGAACTCC164860.13396270723956052Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC156510.1271776253188379No Hit
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA156180.12690947238065364TruSeq Adapter, Index 6 (100% over 40bp)
AAAAGCTGGGTTGAGAGGGCGATAGATCGGAAGAGCACACGTCTGAACTCC146520.11905990455380569Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCCA140160.11389186610880021Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA137590.11180352352960773TruSeq Adapter, Index 6 (100% over 37bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACAGATCGGAAGAGCACA134100.10896760306214404No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG131770.10707428080163103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)