FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12395715
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC4268293.443359257614426No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT3440462.775523638612214Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1897411.5306983098594957TruSeq Adapter, Index 7 (100% over 37bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1413381.1402165990424917Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT1062010.8567557418027116No Hit
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT1024930.8268421789303805TruSeq Adapter, Index 7 (100% over 38bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA994150.8020110175169404TruSeq Adapter, Index 7 (100% over 37bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC837670.6757738460427656Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG774800.6250547064045923TruSeq Adapter, Index 7 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC772630.6233041014576408TruSeq Adapter, Index 7 (100% over 50bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT648770.5233824753150585Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT524300.422968743634393No Hit
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT511630.41274746958928954Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA379480.30613804851111853Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC329490.2658095963000117Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA327690.26435748159747136TruSeq Adapter, Index 7 (100% over 35bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC317880.2564434564686265No Hit
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA303960.24521376943564774TruSeq Adapter, Index 7 (100% over 37bp)
GCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT296650.23931657028255332Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT288020.2323544870142626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG283890.22902269050232277Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG280140.22599745153869705TruSeq Adapter, Index 7 (100% over 36bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA274080.22110866537347787TruSeq Adapter, Index 7 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT271560.21907570478992133Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG243240.19622910013661982No Hit
GGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC234150.188895920888791Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA229740.18533823986756714TruSeq Adapter, Index 7 (100% over 40bp)
GAGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA228220.18411200967431085TruSeq Adapter, Index 7 (100% over 35bp)
AGAAGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC218270.1760850422908239Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT207360.16728361373264874Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC201640.1626691159001316No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC192670.15543274429913884No Hit
GAGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC176840.14266220222068673Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG176070.14204101982015557Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT173390.13987898237415106No Hit
AGGGGCCCGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA168700.13609541684364315TruSeq Adapter, Index 7 (100% over 37bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA165750.13371556219225758Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT150840.12168721207288163No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT141970.11453151351091889No Hit
GTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA134960.10887633347491453Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC133790.10793245891826328Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT127120.10255156721496098Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTAGGATTCGGCGAGATCGGAAGAGCACACGTCT124810.10068802001336752Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG124010.10004263570112737TruSeq Adapter, Index 7 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)