FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12165426
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC6021634.949789674442966No Hit
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC2606242.142333527818919TruSeq Adapter, Index 1 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT2516002.0681561007399165No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT2368971.9472972011008904No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1864341.5324905186222002Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC1473081.2108741609212863No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1182620.9721155675107472TruSeq Adapter, Index 1 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC990770.8144145548211792No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT917390.754096075221698Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC841890.6920349521669031No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC640620.5265906841240084TruSeq Adapter, Index 1 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT616810.5070188253169269No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT592610.48712638587419793Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT541910.44545090324005093No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC491070.4036603403777229TruSeq Adapter, Index 1 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA448120.36835537037502836No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT441160.36263423903116915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA413230.3396757335090444TruSeq Adapter, Index 1 (100% over 37bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA382560.31446494352109Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG381270.3134045614185644No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT341270.28052449622397113No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC315680.25948947451573007No Hit
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC312400.2567933091697734TruSeq Adapter, Index 1 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC309930.2547629651440073No Hit
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA260230.21390948413972516TruSeq Adapter, Index 1 (100% over 40bp)
GGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA256980.21123797884266443Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT243450.20011629679059328Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC238260.1958501083315948No Hit
AAGGCCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA235780.19381154428953TruSeq Adapter, Index 1 (100% over 40bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT218170.17933609558761032Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT206990.17014611736572152No Hit
CGCGACCTCAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCCA206020.16934877578475263Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGCACGTGTTAGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC204400.1680171331443716Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA197470.1623206618494083TruSeq Adapter, Index 1 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTCC185670.1526210426170033Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC184880.1519716613294101Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT183990.1512400798788304Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTAGGATTCGGCGAGATCGGAAGAGCACACGTCT183380.15073865888461285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCCCGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC157600.1295474568666975TruSeq Adapter, Index 1 (100% over 38bp)
AGGGGCCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG147330.12110550012798565TruSeq Adapter, Index 1 (100% over 39bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA144350.11865593527098846TruSeq Adapter, Index 1 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG141440.1162639105280818No Hit
CGGCCGGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC132620.10901385615267399TruSeq Adapter, Index 1 (100% over 38bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAGT127780.1050353682641282Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA127090.10446818713952147Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)