FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15879206
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC13840418.716059228654128No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT5073163.194844880783082No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT3742102.3566039762945326Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG2104161.3251040385772437TruSeq Adapter, Index 5 (100% over 37bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG1937441.2201113834029231Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG1180730.7435699240881439TruSeq Adapter, Index 5 (100% over 39bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1153580.726472091866558No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC764900.48169914792968865No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC757120.4767996586227296No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT730170.45982777728307067No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG719580.45315867808503774Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG711370.4479883943819357Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG651620.41036056840625407TruSeq Adapter, Index 5 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC645150.40628605737591666TruSeq Adapter, Index 5 (100% over 50bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC633610.39901869148873065Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA587200.3697917893375777Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT567190.3571904036007846TruSeq Adapter, Index 5 (100% over 38bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA543850.3424919356799074Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC543480.34225892654834256Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT502600.3165145662824703Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT496100.3124211626198439No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC488660.30773578981216065Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGCCCGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG477200.30051880427774535TruSeq Adapter, Index 5 (100% over 39bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT470460.2962742595568066Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC457170.28790482345275953Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT420350.26471726609000473Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAGTGTGACAATGGTGTTTGAGATCGGAAGAGCACACGTCTGAACTCCA379060.2387147065161822Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC371770.23412379686994425No Hit
AGTGGGTTATCAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA346650.21830436609991707Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG333300.20989714473129195TruSeq Adapter, Index 5 (100% over 37bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA299200.18842251936274396TruSeq Adapter, Index 5 (100% over 36bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC288530.18170303981194022No Hit
AGCGCCCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA277670.17486390692330586TruSeq Adapter, Index 5 (100% over 40bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA276680.17424045005776737Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA264100.1663181395845611TruSeq Adapter, Index 5 (100% over 40bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT263600.16600326237974367Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA262170.16510271357396583Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAGCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT250370.15767161154027473Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGAAGAGGGGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG250000.15743860240870985TruSeq Adapter, Index 5 (100% over 37bp)
TGGAGTGTGACAATGGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCAG228910.1441570819095111Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT215230.13554204158570649No Hit
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA191960.12088765647350379Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGAAACTCTGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC191600.12066094488603522TruSeq Adapter, Index 5 (100% over 35bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC179620.11311648705860985No Hit
TGGAGTGTGACAATGGTGTTTGAAGATCGGAAGAGCACACGTCTGAACTCC171540.10802807142876035Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGAGTGTGGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG169330.10663631418346736TruSeq Adapter, Index 5 (100% over 37bp)
GGGACGGCCGGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC166400.10479113376323729TruSeq Adapter, Index 5 (100% over 35bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT165960.10451404182299795Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT162310.10221543822783079Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)