FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15079026
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC170246711.290298193No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT13249508.786708107009034No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT2868641.9024040412159247Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1817971.2056282680326964No Hit
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA1789651.1868472141370405TruSeq Adapter, Index 9 (100% over 38bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG1240670.8227786065227289Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1228560.8147475838293535Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC1164020.7719464108623462Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC1053940.6989443482622817TruSeq Adapter, Index 9 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC877810.5821397217565644No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC872810.5788238577213144Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC711920.47212598479503914TruSeq Adapter, Index 9 (100% over 37bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC656110.4351143104335784TruSeq Adapter, Index 9 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC595250.3947536133965151TruSeq Adapter, Index 9 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT500040.33161293043728424No Hit
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT480680.3187739048927961TruSeq Adapter, Index 9 (100% over 36bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG461690.3061802532869165Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA412430.2735123608116333TruSeq Adapter, Index 9 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC395810.26249042875846224No Hit
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA372320.24691249952085767TruSeq Adapter, Index 9 (100% over 38bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG364500.2417264881697266Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT354610.23516770910800205Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT354030.23478306887991304Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT317460.2105308393260944Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT315630.20931723308919287No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT309090.20498008293108588Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT294030.1949927004569128TruSeq Adapter, Index 9 (100% over 36bp)
AGAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC272310.18058858708778672TruSeq Adapter, Index 9 (100% over 37bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT262170.17386401482429964TruSeq Adapter, Index 9 (100% over 36bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT256960.17040888449956915TruSeq Adapter, Index 9 (100% over 36bp)
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG250350.1660253122449686TruSeq Adapter, Index 9 (100% over 39bp)
AATCTGGAGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC250240.1659523632361931TruSeq Adapter, Index 9 (100% over 37bp)
AGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG243570.16152900061316958TruSeq Adapter, Index 9 (100% over 39bp)
GGGACGGCCGGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA230770.15304038868292952TruSeq Adapter, Index 7 (97% over 35bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA219630.1456526436123925Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC209510.13894133480504642TruSeq Adapter, Index 9 (100% over 37bp)
AGGGACGGCCGGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG191810.12720317612026133Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC188550.12504123276927834No Hit
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC187570.12439132341836932TruSeq Adapter, Index 9 (100% over 37bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG181090.12009396362868531TruSeq Adapter, Index 9 (100% over 39bp)
AGGGGCCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG175330.11627408826007729TruSeq Adapter, Index 9 (100% over 39bp)
AGCGGGGCTGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT175090.11611492678638528TruSeq Adapter, Index 9 (100% over 36bp)
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC174480.11571039137408477TruSeq Adapter, Index 9 (100% over 37bp)
GAAGAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA174220.11553796644425177TruSeq Adapter, Index 7 (97% over 35bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG168400.11167830070722075No Hit
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG168150.11151250750545824Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGTCTGAACTCCAG167090.11080954432998524Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGCTGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG161790.10729472845262021TruSeq Adapter, Index 9 (100% over 39bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA161410.10704272278594122Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA159290.1056367964349952Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA157180.10423750181211969TruSeq Adapter, Index 9 (100% over 40bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC156240.1036141193734927Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)