FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16008159
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC5545273.4640273125722953No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT3838252.3976835812287973Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT3700522.311646204913382No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1177760.7357248263213777Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT851150.5316976174462035Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG845840.5283805589387262Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG745980.46599986919170405No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC643000.4016701733159947TruSeq Adapter, Index 10 (100% over 50bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG618760.38652789493157835TruSeq Adapter, Index 7 (97% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT514910.3216547261930619No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC490520.3064187455909202Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT485170.3030766998253828Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC451290.281912492248484Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT405240.253145911406802TruSeq Adapter, Index 10 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG366420.22889577745948175No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT359340.2244730327828453No Hit
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT338340.21135472230129648TruSeq Adapter, Index 10 (100% over 38bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC330520.2064697133505483TruSeq Adapter, Index 10 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA305910.19109630282907608No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC300620.18779173795062878No Hit
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC273030.1705567767036797TruSeq Adapter, Index 10 (100% over 37bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG268320.16761452706710372TruSeq Adapter, Index 7 (97% over 36bp)
AGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT265270.16570924864002162TruSeq Adapter, Index 10 (100% over 39bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT264670.16533443976912024TruSeq Adapter, Index 10 (100% over 38bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC248110.15498971493224176TruSeq Adapter, Index 10 (100% over 37bp)
AGAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC233710.1459943020306083TruSeq Adapter, Index 10 (100% over 37bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG228690.1428584011440666TruSeq Adapter, Index 7 (97% over 36bp)
AACGGGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT221860.13859182683030571TruSeq Adapter, Index 10 (100% over 39bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA215750.1347750231616265Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT215000.13430651207299976TruSeq Adapter, Index 10 (100% over 39bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG211570.1321638546943468Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT207190.12942774993676662Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT198970.12429286840541752No Hit
GGGGGCGGGGAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA190830.11920796139018859TruSeq Adapter, Index 7 (97% over 35bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG177360.11079350223845229Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC175290.10950041163384247TruSeq Adapter, Index 10 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT173270.10823855510180777No Hit
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT171040.1068455154649576Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)