FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19314243
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC10822495.60337259917461No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT6869703.556805203289614No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT3845851.991198930240238Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1814160.9392861009359778Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG1687930.8739301871680915No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC1167400.6044244136309148Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1032130.5343880161391777TruSeq Adapter, Index 9 (100% over 50bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA954890.49439680343671766Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT949300.4915025662667701Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT871260.45109715146485424No Hit
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT837160.4334417869755496TruSeq Adapter, Index 9 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG784050.40594394509792586No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG748310.3874394663047369Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC706290.3656835010308196Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG696260.36049044220889215Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT610360.3160154917798228No Hit
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC519650.2690501512277753TruSeq Adapter, Index 9 (100% over 37bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC490760.2540922779111767TruSeq Adapter, Index 9 (100% over 37bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC485700.2514724496321186Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG451300.2336617593555181Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT441320.22849458816480667No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC362340.1876024858960302No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA345950.17911652038342896No Hit
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG339900.17598411700629427TruSeq Adapter, Index 9 (100% over 39bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA328880.17027848308629026TruSeq Adapter, Index 9 (100% over 40bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG325680.16862167468846695Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT317300.16428290769666717Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT311530.16129547505434202Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA311130.16108837400461412TruSeq Adapter, Index 9 (100% over 38bp)
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA307550.1592348196095493TruSeq Adapter, Index 9 (100% over 40bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA306760.15882579503633665No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC303090.15692564290508304No Hit
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC302960.1568583350639215TruSeq Adapter, Index 9 (100% over 37bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA294420.1524367276522305TruSeq Adapter, Index 9 (100% over 38bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC277750.14380579140481975Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG272810.14124809344068TruSeq Adapter, Index 9 (100% over 39bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC265580.137504741966848TruSeq Adapter, Index 9 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT255550.13231168314492056No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT248870.12885309561446442Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA236630.12251580349279026Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTAGTATTCTCTGATGGTAGTTTGTATTTCTGTGAGATCGGAAGAGCACA234230.12127319719442278No Hit
AAAAGCTGGGTTGAGAGGGCGAAAGATCGGAAGAGCACACGTCTGAACTCC223000.11545883522331161Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT218690.11322732141249335No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC216170.11192258479920751No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA208410.10790482443448599Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC205140.1062117733529603TruSeq Adapter, Index 9 (100% over 37bp)
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT199390.10323469576312154TruSeq Adapter, Index 9 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)