FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001839320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8790324
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC5604786.376078970467983No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT3505123.987475319453526No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1253391.425874632152353Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC1163281.3233641899889015Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1036261.1788643968072168Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC768560.8743249964392666TruSeq Adapter, Index 12 (100% over 50bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC544740.6197041201211696Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT453420.5158171644185129No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT414830.47191662104832544No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG405730.4615643291419065Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT335430.3815900301285823TruSeq Adapter, Index 12 (100% over 36bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG315520.35894012552893384TruSeq Adapter, Index 12 (100% over 37bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA307930.3503056315102834TruSeq Adapter, Index 12 (100% over 39bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA296880.3377349913382032No Hit
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA296340.33712067951078933TruSeq Adapter, Index 12 (100% over 40bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT259070.2947217872742802No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA257420.29284472335718226Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT236050.268533901594526TruSeq Adapter, Index 12 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC228670.26013830661986975No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT227990.25936472876312633Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC219630.24985427158316348No Hit
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC216040.24577023554535646Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT215650.24532656589222424TruSeq Adapter, Index 12 (100% over 38bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG212410.241640694927741Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA209440.2382619798769647TruSeq Adapter, Index 12 (100% over 40bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG205830.2341551915492535No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT205390.2336546411713607Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG193670.2203217992874893TruSeq Adapter, Index 12 (100% over 37bp)
ACGGCCCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA182520.20763739766588807TruSeq Adapter, Index 12 (100% over 39bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC175020.19910528895180657No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG164350.18696694228790658No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA156150.17763850342717744Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT152060.17298566014176497Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG142000.16154125831994362TruSeq Adapter, Index 12 (100% over 37bp)
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT140350.15966419440284568TruSeq Adapter, Index 12 (100% over 41bp)
GGGATTGTGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT127670.14523924260357185TruSeq Adapter, Index 12 (100% over 38bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC124250.14134860102995064Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA116620.13266860243149173TruSeq Adapter, Index 12 (100% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT106290.12091704469596343No Hit
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT106050.1206440172171128TruSeq Adapter, Index 12 (100% over 38bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAAC105170.11964291646132726Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA93520.1063897075921206TruSeq Adapter, Index 12 (100% over 39bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA91670.10428512077598051Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG89640.10197576335070242Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)