FastQCFastQC Report
Wed 17 Jul 2024
EGAF00001839336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4144131
Total Bases211.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC1069032.5796240514597635Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG508061.2259747580373304TruSeq Adapter, Index 9 (100% over 49bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAA501761.210772535906804Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT359570.8676607954719578No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG310780.7499280307499931No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT244660.5903770899134221TruSeq Adapter, Index 9 (100% over 36bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG207230.5000565860490415Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA171020.41268000456549275TruSeq Adapter, Index 9 (100% over 35bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT141410.34122956055201925TruSeq Adapter, Index 9 (100% over 36bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCC115730.27926240748663594Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG115200.2779834903867663Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAAC105080.253563412932651Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT98660.23807162466630521TruSeq Adapter, Index 9 (100% over 36bp)
ATCGGACGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA96730.23341443598187414TruSeq Adapter, Index 9 (100% over 38bp)
TGAATTGCAGAATTCCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGT88790.21425480999514734Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGACGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC81600.19690497235729276TruSeq Adapter, Index 9 (100% over 37bp)
AAGGACGCCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC76440.1844536285170522TruSeq Adapter, Index 9 (100% over 37bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC69540.16780357570742818Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC63810.15397679272204473Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC63030.15209461283921769TruSeq Adapter, Index 9 (100% over 37bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA62270.15026069397902722TruSeq Adapter, Index 9 (100% over 35bp)
ACCCTGTTGAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG59530.1436489338778142Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGCTGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG57630.139064136727338TruSeq Adapter, Index 9 (100% over 39bp)
AATTGCAGAATTCCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA56580.13653043303891696Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG56240.13570999565409492No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT54850.1323558545808518No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAA50620.12214864829321273Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA47300.11413731853553857Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCA45970.11092796053020525Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGGACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA45690.11025230621329296TruSeq Adapter, Index 9 (100% over 38bp)
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT44740.10795990763805487TruSeq Adapter, Index 9 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC42520.10260293412539323No Hit
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCC41610.10040705759542833Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)