FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16244028
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC3987182.454551297252135No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT3514722.1636997917019105No Hit
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG2836891.7464202844269905TruSeq Adapter, Index 3 (100% over 37bp)
AGAGAACTCGGGAGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1275800.7853963315010293Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGAACTCGGGAGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1187170.7308347412353635Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAGAAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG1129090.6950800626544106TruSeq Adapter, Index 3 (100% over 38bp)
CTGGCGGAGCGCTGAGAAGACGGAGATCGGAAGAGCACACGTCTGAACTCC997890.6143119182015693Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG990420.6097133050989569TruSeq Adapter, Index 3 (100% over 37bp)
AGAGAACTCGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA982980.605133160322058TruSeq Adapter, Index 3 (100% over 36bp)
AGAGAACTCGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT646680.39810322907594103TruSeq Adapter, Index 1 (97% over 35bp)
AGGGGGGCCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA584910.3600769464322519TruSeq Adapter, Index 3 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT564860.347733948747195No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC541650.33344562075367024TruSeq Adapter, Index 3 (100% over 50bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT511360.31479876789180616Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGAACTCGGGAGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT482350.296939896927043Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGAACTCGGGAGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC472860.2910977498930684Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC421260.25933222966618874Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACAGATCGGAAGAGCACACG382950.23574817773030188No Hit
GGGGCTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG378000.232700903987607TruSeq Adapter, Index 3 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT375590.23121728182197177Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTAAGATCGGAAGAGCAC354980.21852954205693317No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC352600.2170643882170112Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGAACTCGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG334860.20614345161187853TruSeq Adapter, Index 3 (100% over 37bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG324400.19970416204650718TruSeq Adapter, Index 3 (100% over 37bp)
CTGGCGGAGCGCTGAGAAGACGAGATCGGAAGAGCACACGTCTGAACTCCA315660.19432372315536517Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT314000.19330180913256245Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGCCGTAGCTCAGCTGGGAGAGCGCTGCAGATCGGAAGAGCACACGTCT313550.19302478424686292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGAGGGAAAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA305070.1878044041785695TruSeq Adapter, Index 3 (100% over 36bp)
GCGGAGCGCTGAGAAGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGT298090.18350744039594122Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAGATCGGAAGAGCACACGTCT291450.17941978430473035Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACAGATCGGAAGAGCACAC288190.1774128929105515No Hit
AGAACTCGGGAGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT281830.17349760785933144Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGCTGTAGCTCAGCTGGTTAGAGCACCGTGTAGATCGGAAGAGCACACG277480.17081970063090263No Hit
GGGGCTGTAGCTCAGCTGGTTAGAGCACCAGATCGGAAGAGCACACGTCTG273760.16852962824245316Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAACTCGGGAGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT269220.16573475495117346TruSeq Adapter, Index 1 (97% over 35bp)
AGAGAACTCGGGAGAAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC263750.16236736356278136Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGAGAAGGAACTCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA260540.16039125271145802Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGCGGAGCGCTGAGAAGACGGAGATCGGAAGAGCACACGTCTGAACTCCA258000.15882760113439842Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTCTGAA256390.15783646765445122Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG252480.15542942920315084Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGAACTCGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA249130.1533671328318321TruSeq Adapter, Index 3 (100% over 36bp)
GAGAGAACTCGGGAGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC246040.151464895283362Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA242130.1490578568320616Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGAGAACTCGGGAGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA232940.14340039305522004Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGGCGGCGGTCGGCGGGCGGCGGGAGATCGGAAGAGCACACGTCTGAACT228970.14095641795249306Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGAGAACTCGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT228730.14080867134678665TruSeq Adapter, Index 1 (97% over 35bp)
CTCGGGAGAAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG226950.13971288402113072TruSeq Adapter, Index 3 (100% over 37bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA222800.13715809896412393TruSeq Adapter, Index 3 (100% over 36bp)
CTGGCGGAGCGCTGAGAAGACGGTCGAACTAGATCGGAAGAGCACACGTCT215060.13239327093009198Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGAGAACTCGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT213340.13133442025586264Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC213130.13120514197586952No Hit
GGGGCTGTAGCTCAGCTGGTTAGAGCACCGTGAGATCGGAAGAGCACACGT212100.13057106279304617No Hit
GAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG208160.12814555601603247TruSeq Adapter, Index 3 (100% over 38bp)
GAGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT205060.12623716235899127TruSeq Adapter, Index 1 (97% over 35bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA199890.12305445422773219TruSeq Adapter, Index 3 (100% over 36bp)
GGAGAAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC197560.12162008093066573TruSeq Adapter, Index 3 (100% over 39bp)
GAGGGGGGCCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT196650.12105987505069557TruSeq Adapter, Index 1 (97% over 35bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA195030.12006258546217723Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAACTCGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG190310.1171569022166177TruSeq Adapter, Index 3 (100% over 38bp)
GGGGGGCCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG188940.1163135153423769TruSeq Adapter, Index 3 (100% over 37bp)
CTCGGGAGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG188880.11627657869095029TruSeq Adapter, Index 3 (100% over 38bp)
AGGAAACTCTGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT185550.11422659453677377TruSeq Adapter, Index 1 (97% over 35bp)
GCGGCGACTCTGGACGCGAGCAGATCGGAAGAGCACACGTCTGAACTCCAG185040.11391263299964761Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGAAACTCTGGTGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC185000.11388800856536323Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG182070.11208426875403071No Hit
GGCGGCGACTCTGGACGCGAGCAGATCGGAAGAGCACACGTCTGAACTCCA170450.10493087059441168Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAGGAAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA170350.10486930950870067TruSeq Adapter, Index 3 (100% over 40bp)
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG167960.1033979995602076TruSeq Adapter, Index 3 (100% over 37bp)
AGGAAACTCTGGTGGAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC165920.10214215341170306Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGGGGTGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG165530.10190206517743013TruSeq Adapter, Index 3 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)