FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14749634
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC159115510.787759208126792No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT12618398.55505295928021No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC3380552.291955176650485Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC3162002.1437820084213617Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG2629711.7828984773452683Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA1961991.330195718754784Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1820101.2339967215457686No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1669811.1321026677678918TruSeq Adapter, Index 7 (100% over 37bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG1298580.8804150665704655Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC1157310.7846364187748658Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG1045620.7089125058967565TruSeq Adapter, Index 7 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG998450.6769320513309007No Hit
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA993970.6738946878275082TruSeq Adapter, Index 7 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT968180.6564095081952542Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC916310.6212425338825356Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA840030.5695259963738761Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC789890.535531932521173No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG772940.5240401219447208No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT717120.48619511507878765No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT716220.4855849304464097Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT611250.41441706282338936Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG524200.3553986492139398TruSeq Adapter, Index 7 (100% over 36bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT521180.35335114078084917TruSeq Adapter, Index 7 (100% over 38bp)
AAAAGCTGGGTTGAGAGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA472280.32019777575497804Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAGCTGGGTTGAGAGGGCGAAAGATCGGAAGAGCACACGTCTGAACTCC468880.3178926338104389Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGCCCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC443290.3005430507631579TruSeq Adapter, Index 7 (100% over 39bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC435090.2949835907792695Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT431320.2924275951525306No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA419900.28468503015057867Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC410140.2780679168039017No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT399020.2705287466794091No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT397870.269749066315815No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGTCTGAACTCCAG379730.2574504560587741Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC351360.23821608048036988TruSeq Adapter, Index 7 (100% over 50bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG346770.23510413885524212TruSeq Adapter, Index 7 (100% over 36bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA336130.22789040053468446TruSeq Adapter, Index 7 (100% over 37bp)
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC329910.22367334674202763TruSeq Adapter, Index 7 (100% over 39bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT320820.21751048195500985TruSeq Adapter, Index 7 (100% over 38bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAAC286540.19426922729065685Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAGCTGGGTTGAGAGGGCGATAGATCGGAAGAGCACACGTCTGAACTCC265240.17982819099104425Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT263290.17850612428755858Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA262540.17799763709391025TruSeq Adapter, Index 7 (100% over 37bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA259220.17574673378336034TruSeq Adapter, Index 7 (100% over 40bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT243200.16488544732703198Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCCA242100.16413966610968111Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC233710.15845138937006842No Hit
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA230050.1559699718650646TruSeq Adapter, Index 7 (100% over 40bp)
AGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC214910.14570531038261694TruSeq Adapter, Index 7 (100% over 39bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC208270.14120350376151705Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCGGGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT187030.12680314643739635TruSeq Adapter, Index 7 (100% over 38bp)
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT178750.12118944781951876TruSeq Adapter, Index 7 (100% over 41bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA168020.11391469103572334TruSeq Adapter, Index 7 (100% over 37bp)
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA160900.10908745261068852Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGCGGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC160570.10886371824548324TruSeq Adapter, Index 7 (100% over 39bp)
GAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA156630.10619246552151734TruSeq Adapter, Index 7 (100% over 35bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG155650.10552804225515021TruSeq Adapter, Index 7 (100% over 36bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT154320.1046263249650805TruSeq Adapter, Index 7 (100% over 38bp)
ACGGCCCTGGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA148090.10040249134317503TruSeq Adapter, Index 7 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)