FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16182756
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT10243416.329830345337964No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC9805436.059184232895806No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC8287345.121093094402462TruSeq Adapter, Index 1 (100% over 50bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2456541.5179985411631987Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC2400571.4834123433610444TruSeq Adapter, Index 1 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1580870.976885519376304No Hit
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1469560.9081024270525986TruSeq Adapter, Index 1 (100% over 37bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1230350.7602845893493049TruSeq Adapter, Index 1 (100% over 37bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC1179490.7288560737120425TruSeq Adapter, Index 1 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG1125720.6956293476834231No Hit
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1077680.6659434276831462Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1046350.646583313744581Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG1031600.6374686734447458No Hit
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC966060.5969687734277153Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA818350.5056926026691622TruSeq Adapter, Index 1 (100% over 37bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC615930.38060884066966094Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT582160.35974094894590264No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT579060.35782532962864916Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC548360.3388545189706871TruSeq Adapter, Index 1 (100% over 36bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA539570.3334228112937005Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC536380.33145157722207513TruSeq Adapter, Index 1 (100% over 36bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA533750.3298263905109859Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACGT524350.3240177383877011No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC518740.3205510853651875No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG494220.30539915450742755Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT491740.30386665905362475Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC488980.30216113991955384Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT456010.28178760156799No Hit
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA445760.27545369898674865TruSeq Adapter, Index 1 (100% over 40bp)
CGGCCGGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC429410.26535035194252454TruSeq Adapter, Index 1 (100% over 38bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC394020.24348139464007243TruSeq Adapter, Index 1 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT376820.23285279713789173No Hit
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT370740.22909571150921387TruSeq Adapter, Index 1 (100% over 41bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA336680.2080486166880351Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGCCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA334790.2068807068462257TruSeq Adapter, Index 1 (100% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT320810.19824188166712767No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT318140.19659197728742867Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG301920.18656896266618617Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA298210.18427639890263436Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC284140.17558195896916445No Hit
ACGGCCCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG274810.16981656276594667TruSeq Adapter, Index 1 (100% over 39bp)
AGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG257090.15886663557183955TruSeq Adapter, Index 1 (100% over 39bp)
AGCGCCCGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC234550.14493822931026087TruSeq Adapter, Index 1 (100% over 38bp)
AGCGTGAACCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA227150.1403654606174622TruSeq Adapter, Index 1 (100% over 37bp)
AGCGTGAACCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC223830.13831389412285522TruSeq Adapter, Index 1 (100% over 36bp)
GAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT223120.13787515550503263TruSeq Adapter, Index 1 (100% over 35bp)
GCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT208490.1288346682110266Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGCCCTGGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT206130.12737632576305297TruSeq Adapter, Index 1 (100% over 35bp)
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA201930.12478097055903209TruSeq Adapter, Index 1 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC196740.12157385305692059No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC193150.11935544229919799No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT171290.10584723640398458Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT169900.10498829741979673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA169520.10475347956800438Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC167330.10340018721162204Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGTGAACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC163990.10133626188271021TruSeq Adapter, Index 1 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)