FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16782898
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC12434707.409149480620093TruSeq Adapter, Index 6 (100% over 50bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA4534762.702012489142221TruSeq Adapter, Index 6 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC3263511.9445449766780445No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1701561.0138654241955114Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA1542360.9190069557712857TruSeq Adapter, Index 6 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT1067960.6363382533815078No Hit
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC924270.5507213354928333TruSeq Adapter, Index 6 (100% over 36bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG784720.46757121445890926Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA732140.4362417027142749TruSeq Adapter, Index 6 (100% over 38bp)
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA684040.40758157500569925TruSeq Adapter, Index 6 (100% over 37bp)
ACCCGGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA571860.3407397220670709TruSeq Adapter, Index 6 (100% over 40bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT536390.3196051122994372Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT530200.31591683391032943No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT476830.2841166048914794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC466400.2779019451825304Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCGGGACGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA463580.27622166326697567TruSeq Adapter, Index 6 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG432830.25789944025161804No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA423710.25246533703535584Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC367870.21919337172876815TruSeq Adapter, Index 6 (100% over 36bp)
GAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC363740.21673253332052667TruSeq Adapter, Index 5 (97% over 35bp)
AGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT360860.21501650072591755TruSeq Adapter, Index 6 (100% over 39bp)
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT307810.1834069419953574TruSeq Adapter, Index 6 (100% over 41bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC306600.1826859699677612No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT264220.15743407366236747Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT249580.14871090797310452No Hit
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA246960.14714979498773095TruSeq Adapter, Index 6 (100% over 40bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT245460.14625602801137205No Hit
AGGGGCCCGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA236350.14082788324161893TruSeq Adapter, Index 6 (100% over 37bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG230110.13710981261996585Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT215630.12848198207484787TruSeq Adapter, Index 6 (100% over 39bp)
AGGGCTGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT213160.12701024578711018TruSeq Adapter, Index 6 (100% over 39bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC212220.1264501518152586No Hit
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT209000.12453153203934147Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA206090.1227976241052052Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG194380.11582028324309662Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA168270.10026277940794254TruSeq Adapter, Index 6 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)