FastQCFastQC Report
Wed 17 Jul 2024
EGAF00001839360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20946126
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG18722838.938564582300327TruSeq Adapter, Index 7 (100% over 49bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG10190574.865133533523096No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT9656954.610375207329509No Hit
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG5300362.530472699343067TruSeq Adapter, Index 7 (100% over 36bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG4539362.1671596933962873TruSeq Adapter, Index 7 (100% over 36bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG2037930.9729388622984508Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC1719740.8210301036096126Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG1297430.619412868995441No Hit
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA916540.4375701740741939TruSeq Adapter, Index 7 (100% over 35bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC812670.38798105196158944Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG663750.3168843727952367TruSeq Adapter, Index 7 (100% over 36bp)
GGGGCTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG614370.29330960770502384TruSeq Adapter, Index 7 (100% over 36bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCC605350.289003322141765Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC580960.2773591641719333Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAA527780.2519702211282411Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG447710.21374358198742813TruSeq Adapter, Index 7 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGT440370.21023935404570754No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC427430.20406160069885954No Hit
ACCCGGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC411920.1966568901571584TruSeq Adapter, Index 7 (100% over 39bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC405320.19350594950111538No Hit
AGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT402650.1922312507811707TruSeq Adapter, Index 7 (100% over 38bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA399500.19072739274078654Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG361820.1727383860862863TruSeq Adapter, Index 7 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG323760.15456796163643818No Hit
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA300010.1432293494271924TruSeq Adapter, Index 7 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT299420.14294767442915218No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTC295730.1411860121532736No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG293160.13995905495842048No Hit
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA287280.13715185328303667TruSeq Adapter, Index 7 (100% over 37bp)
GAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC285780.13643573040666326Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCA272570.13012907494206805Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA270300.1290453423224896TruSeq Adapter, Index 7 (100% over 35bp)
ACCCGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT243250.11613125978522233TruSeq Adapter, Index 7 (100% over 38bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAG237240.11326199412721952Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC216260.10324582216301No Hit
CGCGGGGTGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG211910.10116906582152708TruSeq Adapter, Index 7 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)