FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20946126
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC18714428.934549520040127TruSeq Adapter, Index 7 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT10182134.861104148805369No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC9633414.599136852322955No Hit
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA5290012.5255314514960903TruSeq Adapter, Index 7 (100% over 37bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA4531222.163273533253834TruSeq Adapter, Index 7 (100% over 37bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT2035760.9719028712039638Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA1716200.8193400536213714Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1296670.6190500334047451No Hit
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG915870.43725030585608055TruSeq Adapter, Index 7 (100% over 36bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC811640.3874893142531464Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA662600.3163353452566838TruSeq Adapter, Index 7 (100% over 37bp)
GGGGCTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA613070.29268896787883353TruSeq Adapter, Index 7 (100% over 37bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA604080.2883970047731022Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC580180.2769867802762191Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC526920.2515596440124537Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA446950.21338074639673227TruSeq Adapter, Index 7 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC439370.20976193879479194No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG426510.20362237866801716No Hit
ACCCGGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA411280.1963513443965724TruSeq Adapter, Index 7 (100% over 40bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT404620.19317175882547444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC402090.19196389824065796TruSeq Adapter, Index 7 (100% over 39bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG398910.19044571774274632Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA361180.17243284032570033TruSeq Adapter, Index 7 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT323700.15453931672138324No Hit
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT299920.14318638205461TruSeq Adapter, Index 7 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC298880.14268987019365775No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT295380.14101891681545314Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT292960.13986357190823737No Hit
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT287060.13704682192783527TruSeq Adapter, Index 7 (100% over 38bp)
GAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA285340.13622566769626038TruSeq Adapter, Index 7 (100% over 35bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG272110.12990946392664687Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG270070.128935536814779TruSeq Adapter, Index 7 (100% over 36bp)
ACCCGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC242980.11600235766747512TruSeq Adapter, Index 7 (100% over 39bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT236980.11313786616198146Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG215990.10311692004526278No Hit
CGCGGGGTGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA211520.10098287387367TruSeq Adapter, Index 7 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)