FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16172293
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC14638229.051418991728632No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA6701844.14402583480277Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC6580174.068792223836162TruSeq Adapter, Index 8 (100% over 50bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT3968532.453906814574779TruSeq Adapter, Index 8 (100% over 36bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT3655572.2603906570329886TruSeq Adapter, Index 8 (100% over 37bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2146471.327251491176916Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA1989821.2303882943500961TruSeq Adapter, Index 8 (100% over 40bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1813761.1215230889027301Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT1718911.062873397111962TruSeq Adapter, Index 8 (100% over 37bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG1676951.0369277875437948TruSeq Adapter, Index 8 (100% over 38bp)
ACGGCCCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA1493070.9232271515239057TruSeq Adapter, Index 8 (100% over 39bp)
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT1440610.8907889561486425TruSeq Adapter, Index 8 (100% over 41bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT1200040.7420345401854889No Hit
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT879010.5435283666948156TruSeq Adapter, Index 8 (100% over 36bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA813090.5027672946563607Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT778280.4812428268520735Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG768840.4754056830407413TruSeq Adapter, Index 8 (100% over 38bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT757540.46841842402929507TruSeq Adapter, Index 8 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC687970.42540040549599245No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT644300.39839743195352695Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTCGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG611830.378319883271964TruSeq Adapter, Index 8 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC600040.3710296369228532No Hit
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG593520.3669980503073992Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA565110.3494309681379134Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCCCTGGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC553030.34196140275222564TruSeq Adapter, Index 5 (100% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT547690.33865945911318823Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA547230.33837502202068687Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCGCCCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA479770.29666170406385783TruSeq Adapter, Index 8 (100% over 40bp)
AGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA465640.28792453859202277TruSeq Adapter, Index 8 (100% over 39bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT465200.2876524683296302No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC461000.28505543400679173Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG443900.27448179426380664Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT438090.27088923011721344TruSeq Adapter, Index 8 (100% over 37bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC418430.2587326361203077Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA415870.25714968186638715TruSeq Adapter, Index 8 (100% over 39bp)
GAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC407510.25198034688092774TruSeq Adapter, Index 5 (100% over 35bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAGATCGGAAGAGCACACGTCTG407140.2517515605239158Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGCCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA400010.2473427855901448TruSeq Adapter, Index 8 (100% over 40bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA386580.2390384591721162TruSeq Adapter, Index 8 (100% over 39bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT371160.22950363315826644Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT360420.22286264538986528TruSeq Adapter, Index 8 (100% over 36bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT354030.21891144317011815Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG347860.21509627608156742Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACGGGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA336130.20784313022278286TruSeq Adapter, Index 8 (100% over 39bp)
AGAAGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA311920.19287308237613554Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC300870.18604040874104866No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC274790.16991406227923275No Hit
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA261240.16153553488055156TruSeq Adapter, Index 8 (100% over 40bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACAGATCGGAAGAGCACA256230.15843764393830856No Hit
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT254270.15722569458765062TruSeq Adapter, Index 8 (100% over 37bp)
AGCGCCCGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG230350.1424349657775802TruSeq Adapter, Index 8 (100% over 38bp)
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA228380.14121683301186788TruSeq Adapter, Index 8 (100% over 39bp)
ACGGCCCTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA225920.1396957129084911TruSeq Adapter, Index 8 (100% over 39bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT222050.1373027312824471No Hit
ACGGCCCTGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT215400.1331907602712862TruSeq Adapter, Index 8 (100% over 37bp)
GGGACGGCCGGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC212230.13123061769904862TruSeq Adapter, Index 5 (100% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG208450.12889328680849402No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACAGATCGGAAGAGCACACGTCTGAA193990.11995206863986449Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAAAGATCGGAAGAGCACAC186290.11519083904799399No Hit
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG172380.10658970870735522TruSeq Adapter, Index 8 (100% over 38bp)
AGAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT169400.1047470510211508TruSeq Adapter, Index 8 (100% over 37bp)
AGGGCGGAGGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC165630.10241590354565057TruSeq Adapter, Index 5 (100% over 35bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT162850.1006969141605337No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)