FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13532387
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC157289511.623189611707083TruSeq Adapter, Index 10 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC7614955.627203833292678No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT4341193.20800018503757No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC2029191.499506332474825Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1869611.3815818303156715Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA1630691.2050276126451305TruSeq Adapter, Index 10 (100% over 40bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC1628971.203756587806719TruSeq Adapter, Index 10 (100% over 37bp)
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT1222660.9035065284491198TruSeq Adapter, Index 10 (100% over 41bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1208280.8928801696256544Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG925590.6839813256892521Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT821650.6071729991168594Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT724770.5355817861253894TruSeq Adapter, Index 10 (100% over 39bp)
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA663240.4901130894349977TruSeq Adapter, Index 10 (100% over 40bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA648510.47922809183627396Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT647050.47814919865948263No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC626060.46263826182328366Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG565070.4175686078147189TruSeq Adapter, Index 7 (97% over 36bp)
AGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT552020.4079250763372345TruSeq Adapter, Index 10 (100% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT551930.40785856922359676Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA549770.4062623984962889Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC531770.39296097576872435TruSeq Adapter, Index 10 (100% over 37bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT518620.3832435474983091Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT456910.3376418365806417Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG430370.31802962773677695TruSeq Adapter, Index 7 (97% over 36bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG415710.3071963578931049TruSeq Adapter, Index 7 (97% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC407050.3007968956252877No Hit
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG390840.28881822549118646Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT383980.28374890549612575TruSeq Adapter, Index 10 (100% over 38bp)
ACGGCCCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT347360.25668789992482477TruSeq Adapter, Index 10 (100% over 39bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT343600.2539093805106224TruSeq Adapter, Index 10 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG343450.25379853532122604No Hit
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC324230.23959557171990428TruSeq Adapter, Index 10 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG322970.23866447212897474No Hit
AAGGCCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA292150.21588948054766685TruSeq Adapter, Index 10 (100% over 40bp)
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC282750.20894318201216092TruSeq Adapter, Index 10 (100% over 37bp)
GAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA260740.19267849788806662TruSeq Adapter, Index 7 (97% over 35bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT257220.1900773307768984Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT233600.1726229082866164TruSeq Adapter, Index 10 (100% over 39bp)
AGCGCCCGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT225400.1665633712662814TruSeq Adapter, Index 10 (100% over 38bp)
AGCGCCCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA210130.15527933098573077TruSeq Adapter, Index 10 (100% over 40bp)
AGAAGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT204080.15080857501341044Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAAC202370.1495449398542918Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT201220.14869512673558627TruSeq Adapter, Index 10 (100% over 38bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC200800.1483847602052764TruSeq Adapter, Index 10 (100% over 37bp)
AGAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC198160.1464338848719003TruSeq Adapter, Index 10 (100% over 37bp)
AGCGCCCGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT194610.14381054872285282TruSeq Adapter, Index 10 (100% over 39bp)
AAAAGCTGGGTTGAGAGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA193290.14283511105616473Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCGGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA187830.13880034616213682TruSeq Adapter, Index 10 (100% over 40bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT185680.13721156511412214No Hit
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT185200.13685686050805373TruSeq Adapter, Index 10 (100% over 38bp)
AACGGGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT185170.1368346914701745TruSeq Adapter, Index 10 (100% over 39bp)
ACCCGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT184920.1366499494878472TruSeq Adapter, Index 10 (100% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT162610.12016357498496016No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA155820.11514598274495107Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA152430.11264088146459306TruSeq Adapter, Index 7 (97% over 35bp)
GAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA145220.10731292269427412TruSeq Adapter, Index 10 (100% over 40bp)
AAGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT138510.10235444788860974TruSeq Adapter, Index 10 (100% over 38bp)
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGTCTGAACTCCAG137330.10148246573202496Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)