FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18991235
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC7495213.946668028698502No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC3553041.8708841210168796TruSeq Adapter, Index 9 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT2032041.0699883393576037No Hit
CGGATGCTGACGCAATGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1106630.5827056534238031Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT566610.29835342461930464Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTTGAAGACTGAAGTGGGGAAAGGTTCCATGTGAGATCGGAAGAGCACA532450.28036617945067815No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC429770.22629913220493558No Hit
GGCTGGTCCGATGGTAGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC413270.2176109136662255Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC344130.18120464519553364No Hit
AAATGGATTTTTGGAGCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT324780.1710157343637736Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG311930.16424945507756605Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT289240.15230183819009138No Hit
GGCTGGTCCGATGGTAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGT277620.14618322610404222Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT260120.13696844886601633Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGAACTTTGAAGACTGAAGTGGGGAAAGGTTCCATGTGAGATCGGAAGA259090.13642609340572112No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT233600.12300411216016231Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA230150.12118748464752292Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT212130.11169889688585287TruSeq Adapter, Index 9 (100% over 51bp)
GGCTGGTCCGATGGTAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA200680.10566979977868739Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)