FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26390577
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC13046524.9436281745563955No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT6266592.37455588788377No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT2427710.9199154683127996No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT1996640.7565730753063868No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1877160.7112993399121209No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1858790.704338522041409TruSeq Adapter, Index 3 (100% over 50bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC1358540.5147822270047373No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA1239810.46979268395685325Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1199090.4543629341639631No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1139020.43160102183442217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1044110.3956374277076246No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT870820.32997383876828457Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC772490.29271432754198595No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT694830.26328715738197006Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC586990.22242408720354995No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT581160.22021496536434199No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC568790.21552768626468458No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT552140.20921861617500823Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT455620.17264495581131098Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT370050.14022050370478828Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT327430.1240707999677309No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT324930.12312349214645818Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC320640.12149791192515419No Hit
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC299330.11342306005662552Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC287930.10910333639162191Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA283910.10758006541501537Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT267570.10138846149517686Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT267260.10127099532533904Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)