FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12553664
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC8783316.996610710625998No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT2108621.6796849111143966No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1738061.384504157511305Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1423551.1339717233152011TruSeq Adapter, Index 7 (100% over 50bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1161600.9253075436780847Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG937040.7464274971832925Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC581480.4631954463653002Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC573840.4571095737467563Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA545890.43484515755718806Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC491780.39174220371040674No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT445580.3549401991323011No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC430610.34301539375277207No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA418000.33297051761143204Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT368200.29330082436490257Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG351900.280316567338428Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC337720.26902106030558093No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT335510.26726061809524293No Hit
AAATGGATTTTTGGAGCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT320080.2549693858303042Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT288550.22985321257602562Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT282790.22526491070654753Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT280590.22351243429806628No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC280320.22329735764793449No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC269960.2150447869243593Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC263090.20957228104878384Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC260580.2075728647827439Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG254910.2030562551299764TruSeq Adapter, Index 7 (100% over 36bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC241950.19273257592365065No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA219540.17488121396271242Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA211840.16874754653302812Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT209330.1667481302669882Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC181650.14469879072755173Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT173730.13838987565701932Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA165720.13200926836977633TruSeq Adapter, Index 7 (100% over 37bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG159010.12666421532390862Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)