FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16794687
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT5644483.360872399705931Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA1356550.8077256813419624Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT935210.5568487224560958Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC765690.4559120393252938TruSeq Adapter, Index 4 (100% over 50bp)
CCGTGGACGGTGTGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA276620.16470685044621552Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGTTGTTTGGGAATGCAGCAGATCGGAAGAGCACACGTCTGAACTCCAG183100.10902257362700478Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGGATCTTCGGTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA178390.1062181152884838Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)