FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001839394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875053
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT34873839.853357453777086Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC803189.178644036418365TruSeq Adapter, Index 9 (100% over 50bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT748528.553996157947005Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCGGATGGTGGAATCGGTAGACACAAGATCGGAAGAGCACACGTCTGAA87921.0047391415148568Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCTG87591.0009679413704085Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTGTCGGTTGCTGTCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGT64360.7354983069596926Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGCAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC43440.4964270735601158TruSeq Adapter, Index 9 (98% over 50bp)
CTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC35170.40191851236439396Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCAGATCGGAAGAGCACACGTCTGA29030.33175133391920264Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGTCGGTTGCTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC23250.2656981919952277Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCTGTAGTTCAGTCGGTAGAACGAGATCGGAAGAGCACACGTCTGAAC19910.2275290753817197Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGTCGGTTGCTGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGT19870.22707196021269568Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCGGATGGTGGAATCGGTAGACAAGATCGGAAGAGCACACGTCTGAACT18980.21690114770191063Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGTCGGTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC16340.18673154654632348Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT14480.16547569118670527Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGTCGGTTGCCGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT13620.15564771505268823Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGTCGGTCGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT12620.14421983582708706Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGCCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT12080.1380487810452624Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGTCGGTTGCTGTCGGTTAGTTCGGAAGAGCACACGTCTGAACTCCAGT11560.13210628384794978Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GCTGTCGGTTGCTGTCGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT11510.13153488988666973Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGTCGGCTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT11240.1284493624957574Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGTCGGTTGCTGCCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT10680.12204975012942074Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGAA10290.11759287723143627Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCAGATCGGAAGAGCACACGT10280.11747859843918025No Hit
GCTGTCGGTTCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC10110.11553585897082805Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCTGTCGGTTGCTGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC10110.11553585897082805Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCCGGACGGTGGAATCGGTAGACACAAGATCGGAAGAGCACACGTCTGAA9130.10433653732973888Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGAAGGTGGCGGAATTGGTAGACGCGAGATCGGAAGAGCACACGTCTGAA9080.10376514336845884Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)