FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18870110
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC12356466.548165326010288No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT10637835.637396920314719No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG2444791.295588631968759No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT2363791.2526636039747516Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC2250851.1928123365470578Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG1912461.0134864078693766Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG1579210.8368843636841544No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1454360.7707215273254898No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1306370.6922959113645867Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT1193170.6323068598964182No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC1079700.572174725001603Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA1049590.5562182732374109Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA950000.5034416863494701TruSeq Adapter, Index 5 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC901630.477808555435024TruSeq Adapter, Index 5 (100% over 50bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT783230.4150638231573637No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT781050.413908556971846No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC637450.33780937154049445No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT625520.33148720383717956Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC569960.30204381426499366No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA559440.29646886001194483No Hit
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG553700.2934270123491596Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT545560.28911331200507046Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACAGATCGGAAGAGCACA499990.2649640092188122No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG498710.2642856877887834TruSeq Adapter, Index 5 (100% over 37bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC489970.2596540242743683Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT478980.2538299988712307TruSeq Adapter, Index 5 (100% over 38bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC433780.22987677337334014No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT418900.22199128674925583No Hit
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA412120.21839830292457227Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA387960.2055949859327794Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG381570.2022086781688077TruSeq Adapter, Index 5 (100% over 37bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAAAGATCGGAAGAGCACAC372110.1971954588500014No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT350500.18574348533209398Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA332930.17643246382771485Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG332330.17611450065738884No Hit
AGCGCTGAGAAGACGGTCGAACAGATCGGAAGAGCACACGTCTGAACTCCA326430.17298786281585005Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT317120.16805413428962523Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA303130.16064029303485777No Hit
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT299890.1589232919150975Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC296370.1570579079825184No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACAGATCGGAAGAGCACACGTCTGAA278070.14736003128757597Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT276340.1464432374798027Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG267560.1417903764206992Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGTCTGAACTCCAG261520.138589547172751Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC259190.13735479019465177No Hit
AAAAGCTGGGTTGAGAGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA255640.13547350810355635Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAGCTGGGTTGAGAGGGCGAAAGATCGGAAGAGCACACGTCTGAACTCC247240.13102202371899263Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC246890.13083654520296914Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGA246080.13040729492302905No Hit
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC242620.12857370730748258Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG232210.1230570463023268No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAAC231920.1229033641033359Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAAGAACTTTGAAGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAG225660.11958594835960151Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA219970.1165705976276768Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA200280.10613610625481251TruSeq Adapter, Index 5 (100% over 36bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG200160.10607251362074731TruSeq Adapter, Index 5 (100% over 37bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA199850.10590823264941221Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA195550.10362949659540935Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAG194500.10307306104733889No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)