FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25032748
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC279028011.146518951894533TruSeq Adapter, Index 10 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT13080455.22533522887699No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC8920493.5635280633192967No Hit
GGGGCTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC3717051.4848749326282515TruSeq Adapter, Index 10 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT2715311.084703125681607Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG2574371.0284008771230388No Hit
GGGGCTGTAGCTCAGCTGGTTAGAGCACCAGATCGGAAGAGCACACGTCTG2453570.9801440896540803Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG2361380.9433163310716027No Hit
GGGGCTGTAGCTCAGCTGGTTAGAGCACCGTGTAGATCGGAAGAGCACACG2338570.9342042671463796No Hit
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAGATCGGAAGAGCACACGTCT1873260.7483237557458734Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1630650.6514067093233232No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT1487340.5941577009443789No Hit
AGAGAACTCGGGAGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1412870.5644086697952618Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGAACTCGGGAGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1383180.5525482060539258Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT1207250.48226826715149285No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGA1202030.4801829986863608No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1182750.4724810875737654Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGCTGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC1127530.4504219832357199TruSeq Adapter, Index 10 (100% over 37bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC951450.3800821228256682Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC899120.3591775062010771No Hit
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG837120.3344099497186645Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAAAGAACTTTGAAGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAG807740.32267332375974067Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGG779080.3112243210373867No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC746740.29830524399478636Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCCGTAGCTCAGCTGGGAGAGCGCTGCAGATCGGAAGAGCACACGTCT725740.28991623292816276Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT713780.28513849138736186No Hit
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAAGATCGGAAGAGCACACGTC702960.2808161533044634No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG659840.263590717247663Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAG637330.25459849633767734No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT624770.24958106876640154No Hit
GGGGCTGTAGCTCAGCTGGTTAGAGCACCGTGAGATCGGAAGAGCACACGT607890.24283790177570597No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCCA593530.23710141611300528Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCC568580.22713447201242148Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG558230.22299988798672846Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT530590.21195835151618195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAACTCTGGAGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA520030.20773987737982264Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGCTGTAGCTCAGCTGGTTAGAGCACTAGATCGGAAGAGCACACGTCTG492980.1969340321725765Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGAACTCTGGAGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC486200.19422558002820944Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATACTGGTTGGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT484520.19355445914287955Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT483040.1929632336010413Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA482010.19255177258205933Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA472070.18858097401052415Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAATAGATCGGAAGAGCACACG465920.1861241921981558No Hit
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCCA457250.18266072905779263Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC454130.18141436169932282No Hit
GGGGCTGTAGCTCAGCTGGTTAGAGCACCGAGATCGGAAGAGCACACGTCT451580.18039569606980424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAACTCGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA420250.16788009051183675TruSeq Adapter, Index 7 (97% over 35bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC403020.1609971066700308No Hit
AGAGAACTCGGGAGAAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC383920.15336710136657788Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAA383040.15301556185521462No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC369070.14743487211232265TruSeq Adapter, Index 10 (100% over 50bp)
GAGAACTCGGGAGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT357170.14268109917456925Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCA356310.14233754919755515Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATACTGATTGGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT354060.14143872658327403Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGAACTCGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG348430.139189672663984TruSeq Adapter, Index 7 (97% over 36bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAA345600.1380591535535771No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA340260.13592594788234996Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGAACTCGGGAGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC336980.13461566424908683Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC323060.12905494834206777No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT320900.1281920786323579Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA316260.12633850666335156No Hit
GGGGCTGTAGCTCAGATGGGAGAGCGCCAGATCGGAAGAGCACACGTCTGA304440.12161669186299483Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCCGTAGCTCAGCTGGGAGAGCGCTGCAAGATCGGAAGAGCACACGTC303960.12142494303861485No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT290220.11593613294073826Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG289120.11549670855153418No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG287410.11481360336468054Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG286900.11460987023877683Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGGAAGAGCACACG278540.11127024488082572No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG272410.10882145260280654No Hit
GGGGCTGTAGCTCAGCTGGTTAGAGCACCGTGCAGATCGGAAGAGCACACG267840.10699584400402226No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA267050.10668025739723021Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAATAGTGGAACAGCTTGGAAACGCTAGATCGGAAGAGCACACGTCTGAA265090.10589728303101202Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA264330.10559368072574372No Hit
TGAAAAGAACTTTGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC263390.10521817261133296Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCAG259680.1037361139895628Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC254690.10174272516944605No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA253370.10121541590240113Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTGTAGCTCAGATGGGAGAGCGCTGTAGATCGGAAGAGCACACGTCT251340.10040447816596085Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)