FastQCFastQC Report
Wed 17 Jul 2024
EGAF00001839402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31689698
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT23084237.284458816868498No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG11567603.6502714541489163No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAA7812352.4652648946039184Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA5953461.8786736307805771Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC3219091.0158159285708561No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG2511840.7926361431402722Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC2301080.726128724861941No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2109550.6656895247155716No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA2095050.6611139052192924Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG1759790.555319271266012Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC1759470.5552182920771287Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG1682140.5308160399635238No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCC1584840.5001120553436641Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1540900.4862463504701118Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG1519740.4795691016051967Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAAC1468420.4633745641880209Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACT1322510.41733120965684173Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG1312930.41430814518964487No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT1228430.38764332812512126No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC1166020.36794923069320506Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1142150.3604168143224337TruSeq Adapter, Index 12 (100% over 49bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1045400.32988638768346734No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA1010590.3189017452927447Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC991460.3128650831573087Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGT936460.29550928506797386No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG909650.2870491223993362No Hit
TGGAGTGTGACAATGGTGTTTGAGATCGGAAGAGCACACGTCTGAACTCC787110.24838040425629807Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC783790.24733274517163276Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA774260.2443254587026989Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAAC745490.23524679850215044Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCA708260.223498500995497Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAA699770.22081939689043423Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC664460.20967697451708123Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTC621210.1960290060195588No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGG602980.1902763478528574No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC592250.18689038942561081No Hit
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC590390.18630344789022601Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAGTGTGACAATGGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCA546210.1723620086250112Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCC543650.17155417511394397Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC540800.17065482921295116No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT509490.1607746467006407No Hit
TGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAA490400.15475060696381518No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG485860.15331796472153192No Hit
TGGAGTGTGACAATGGTGTTTGAAGATCGGAAGAGCACACGTCTGAACTC455770.1438227653668394Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC445090.14045258493785584Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCA440660.13905465429175123Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAAATAGGAGAAGATCGGAAGAGCACACGTCTGAACT432690.13653964136862395Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC406420.1282498810812271No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC393240.12409080073909193Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAGCTGGGTTGAGAGGGCGAAAGATCGGAAGAGCACACGTCTGAACTC369200.11650473917422628Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGCACGTGTTAGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA360500.11375936747645876Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC358230.1130430463553171No Hit
TGAAAAGAACTTTGAAGAGAGAGATCGGAAGAGCACACGTCTGAACTCCA357300.11274957558762473Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCG352270.11116230896236372No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT347440.10963815433015488No Hit
GCGGGACATGTGGCGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA346280.10927210477045253Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT336770.1062711295008239TruSeq Adapter, Index 12 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA327170.1032417538343218No Hit
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT321500.10145252883129399TruSeq Adapter, Index 12 (100% over 36bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC317650.10023762296504056Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)