FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001839411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7245369
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG5400747.454057895463985Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC5285147.294507705542672No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT2144222.9594351923276787No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1202751.660025873078376TruSeq Adapter, Index 9 (100% over 50bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT1098561.516223673356043TruSeq Adapter, Index 9 (100% over 36bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG693610.9573149414474267Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC521010.7190938101289251TruSeq Adapter, Index 9 (100% over 37bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC351960.4857723602483186TruSeq Adapter, Index 9 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT350100.4832052032132525No Hit
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA279070.385170168696722Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGGCCCTGGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA267120.36867687484239936TruSeq Adapter, Index 7 (97% over 35bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC261710.3612100363694382Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC238090.3286099024080071No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT237880.3283200620975964Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC232960.32152951768226024Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA214040.2954162859062112Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA204990.2829255487194648TruSeq Adapter, Index 9 (100% over 38bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC198690.2742303394071441Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG187940.25939327589802536Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC177350.24477704310160048TruSeq Adapter, Index 9 (100% over 37bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT170650.23552975700754508TruSeq Adapter, Index 9 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT156290.21571020054327117Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA115400.15927415153044655TruSeq Adapter, Index 9 (100% over 38bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT113110.1561135119550157TruSeq Adapter, Index 9 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC109160.150661753735386No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT101830.14054494671009854Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG101020.13942699122708588Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG95710.1320981719495584Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC91230.12591491199413032No Hit
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC89660.12374801062582182Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA89380.12336155687860757TruSeq Adapter, Index 9 (100% over 38bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC89120.12300270697048003TruSeq Adapter, Index 9 (100% over 37bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG88190.12171912845294697TruSeq Adapter, Index 9 (100% over 39bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA84940.11723350460135294TruSeq Adapter, Index 9 (100% over 40bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG84090.11606034144016682Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG81950.11310673065788644Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC81270.11216820012893752TruSeq Adapter, Index 9 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT77120.10644040351844054No Hit
AGGGCGGAGGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA74440.10274148908081839TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)