FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12331881
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC9589917.776518440293091No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT4186303.3946970458115837No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1579021.280437266626235TruSeq Adapter, Index 9 (100% over 50bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT839510.680763948338457Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC815460.6612616518112687Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC781670.6338611279171442Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT737900.5983677591439619Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG690650.560052436445016Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC675200.547523934102186TruSeq Adapter, Index 9 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT666120.5401609048935844No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG640260.5191908679624787Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT637770.5171717112742168TruSeq Adapter, Index 9 (100% over 36bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA615100.4987884654417278Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC451670.36626204874990276No Hit
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG405670.32896035892659037Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC377230.3058981837401772Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC374100.3033600470195909Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT357960.2902720193294113Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA276510.2242237011531331TruSeq Adapter, Index 9 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT268390.21763914199301793No Hit
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG267690.2170715075826632Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC262450.21282235856800757No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT242580.19670965037693763Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT208880.16938210804985873Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC207240.1680522217170276No Hit
ACGGCCCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG200540.1626191495036321TruSeq Adapter, Index 9 (100% over 39bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC199810.16202718790426215Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGACATGTGGCGTACGGAAGACCCGCAGATCGGAAGAGCACACGTCTGAA187990.15244227543227185Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC181130.14687945821079526TruSeq Adapter, Index 9 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT155860.12638785599698862Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT151270.12266579607766244Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA150820.12230088824243439Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC148770.12063853032639546No Hit
AAAAGCTGGGTTGAGAGGGCGAAAGATCGGAAGAGCACACGTCTGAACTCC139460.11308899266867722Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA138970.1126916485814289TruSeq Adapter, Index 9 (100% over 38bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA136340.11055896501109605Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT134360.10895337053609258No Hit
TGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAG133670.1083938451887429Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT133550.10829653643268208TruSeq Adapter, Index 9 (100% over 36bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG133090.10792351953444897Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA128070.10385276990590488Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT124230.1007388897119588Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA124160.10068212627092331Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)