FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18713262
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC10898955.824185008471532No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT8984714.8012527158546705No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC3221831.721682729606415TruSeq Adapter, Index 10 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1327760.7095288891909919No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA1005230.537175186239577Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC784650.41930156270991126No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC634780.33921397562862105No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT608740.32529871061496385Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT559390.2989270390165007Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT548250.292974041618185Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT473160.2528474191191253No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT439210.2347052053244378Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC399250.21335136546477038TruSeq Adapter, Index 10 (100% over 37bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC396680.21197800789621823No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT375890.20086823986112096No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT368490.19691382507229363No Hit
CGGAGGAGAAGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC349600.18681937975324664TruSeq Adapter, Index 10 (100% over 37bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC343640.18363447270711009Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA342410.18297718484356176TruSeq Adapter, Index 10 (100% over 40bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA338600.18094119560769253Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC337940.1805885045589593TruSeq Adapter, Index 10 (100% over 37bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT313200.16736793403523126Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT275710.14733401370642915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG258080.13791288766223656No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC256760.13720750556477007Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC245270.13106747503455035No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC229230.12249601378957875No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC227740.12169978702804461No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA225700.12060965105923276No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG219830.11747283824701434No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAGATCGGAAGAGCACACGTCTG212570.11359323671094863Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA200170.10696692003777857TruSeq Adapter, Index 10 (100% over 40bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG189340.1011795805562921Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)