FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26898820
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG311869211.594159148988691TruSeq Adapter, Index 3 (100% over 37bp)
ACTGAACAAAGAATAGACGAACCAGATCGGAAGAGCACACGTCTGAACTCC10445993.883438009548374Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGGCTTAGCTCAGTTGGTAGAGCATCTGATTTAGATCGGAAGAGCACAC7346102.7310119923476197No Hit
GCGGGTGTAGTTTAGTGGTAAAACCACAAGATCGGAAGAGCACACGTCTGA4892131.8187154678160604Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGGCTTAGCTCAGTTGGTAGAGCATCTGATTAGATCGGAAGAGCACACG4627201.7202241585318614No Hit
TGTGGTGGCGATAGCGAGAAGGATAGATCGGAAGAGCACACGTCTGAACTC4387301.6310380901467054Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGAACAAAGAATAGACGAACCAAATGTGTAGATCGGAAGAGCACACGTC4197111.5603323863277274No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC3930341.4611570321672103No Hit
CGGGAAGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG3112321.1570470377510984TruSeq Adapter, Index 3 (100% over 37bp)
ACTGAACAAAGAATAGACGAACCAAATGTGAGATCGGAAGAGCACACGTCT2601730.9672283022080521Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGAACAAAGAATAGACGAACCAAATGTGTAAGATCGGAAGAGCACACGT2359020.8769975783324325No Hit
CGCGGGGTGGAGCAGTCAGGTAGCTCGTCGGGCTAGATCGGAAGAGCACAC2234510.830709302489849No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT2096910.7795546421738946No Hit
ACTGAACAAAGAATAGACGAACTAGATCGGAAGAGCACACGTCTGAACTCC2067560.7686433828695831Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGAACAAAGAATAGACGAACAGATCGGAAGAGCACACGTCTGAACTCCA1993890.7412555643704817Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGTTTGGTAGTTCAGCTGGTTAGAATGAGATCGGAAGAGCACACGTCTGAA1974870.7341846222250642Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGAAATAGCTCAGTGGTAGAGCACCACCTTGCCAGATCGGAAGAGCACA1431550.5321980666809919No Hit
ACTGAACAAAGAATAGACGAACCAAATGTGTAGGAGATCGGAAGAGCACAC1389660.5166248928391655No Hit
ACTGAACAAAGAATAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1040100.386671236879536Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCGATCGTGGTGAAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC968280.3599711808919499Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA960450.357060272532401Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGAAGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG941010.34983318970869354TruSeq Adapter, Index 3 (100% over 37bp)
GCGGGTGTGGCGGAATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT896150.33315587821324505Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGGCATAGCTCAGTTGGTAGTAGCGCATGACTAGATCGGAAGAGCACAC850700.3162592262411511No Hit
ACTGAACAAAGAATAGACGAACCAAATGTAGATCGGAAGAGCACACGTCTG734950.2732275988314729Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGAACAAAGAATAGACGAACCAAATGTGTAGGGAGATCGGAAGAGCACA712720.26496329578769623No Hit
TGTGGTGGCGATAGCGAGAAGGAAGATCGGAAGAGCACACGTCTGAACTCC693700.2578923536422787Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGCCGATGAAGGACGGGACTAGATCGGAAGAGCACACGTCTGAACTCCAG669810.24901092315573695Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGGTGTAGTTTAGTGGTAAAACCACAGCCTAGATCGGAAGAGCACACGT648830.24121132451163288No Hit
GCCGGCTTAGCTCAGTTGGTAGAGCATCTGATTCAGATCGGAAGAGCACAC622490.23141907340173284No Hit
CGGGAAGTAGCTCAGCTTGGTAGAGCACTTGGTTTAGATCGGAAGAGCACA618420.22990599587639904No Hit
GGCGATCGTGGTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC606550.22549316289710847Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC603810.22447453085302627Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGTGTGGCGGAATTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC570470.21207993510496Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGAACAAAGAATAGACGAACAAGATCGGAAGAGCACACGTCTGAACTCC556360.20683435184145624Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGAACAAAGAATAGACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT542260.20159248621314987Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTGAACAAAGAATAGACGAACCAAATGTGCAGATCGGAAGAGCACACGTC539760.20066307741380476No Hit
GCGGAAATAGCTCAGTGGTAGAGCACCACCTAGATCGGAAGAGCACACGTC506920.1884543634256075No Hit
GCGGGTGTGGCGGAATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC498710.18540218492855823Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGTGAGAGTAGGACGTCGCCACGAGATCGGAAGAGCACACGTCTGAACTC485210.18038337741209465Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGTGGCGATAGCGAGAAGGACAGATCGGAAGAGCACACGTCTGAACTC480770.17873274738445774Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAAGTAGCTCAGCTTGGTAGAGCACTAGATCGGAAGAGCACACGTCTG465240.172959259922926Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGGCTTAGCTCAGTTGGTAGAGCATCTGATCAGATCGGAAGAGCACACG464410.1726506962015434No Hit
ACTGAACAAAGAATAGACGAACCAAATGAGATCGGAAGAGCACACGTCTGA451690.16792186423047553Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAAGTAGCTCAGCTTGGTAGAGCAAGATCGGAAGAGCACACGTCTGAA440800.16387335950052828Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGGCTCAGCTCAGTTGGTAGAGCATCTGATTTAGATCGGAAGAGCACAC439460.16337519638407932No Hit
GAGCCGTTAGCTCAGTTGGTAGAGCATCTGACAGATCGGAAGAGCACACGT409950.15240445491660973No Hit
GGGCTGTTAGCTCAGTTGGTAGAGCAGATAGATCGGAAGAGCACACGTCTG402830.14975749865607488Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGATCGTGGTGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT401440.149240747363639Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTGGATATTGAAGTAAAAAGAATCAAAACAAACCGAGAACAGATCGGAAG384690.14301370840802682No Hit
AATCTTCCGGACTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT383100.14242260441164334Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGGAAATAGCTCAGTGGTAGAGCACCACCTTGCTAGATCGGAAGAGCACA377010.14015856457643866No Hit
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC347360.12913577621620576Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGTGGCGATAGCGAGAAGGAGATCGGAAGAGCACACGTCTGAACTCCA345420.12841455498791396Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTGAACAAAGAATAGACGAACCAAGATCGGAAGAGCACACGTCTGAACTC341300.12688288928659325Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACCCATAGCTCAACTGGATAGAGTGTTTGACTAGATCGGAAGAGCACAC340190.12647023177968403No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC323950.12043279221913823No Hit
TTTGTGAGAGTAGGACGTCGCCACGAGATCGGAAGAGCACACGTCTGAACT318160.11828028143985499Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGGGGTGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG315380.11724677885498323TruSeq Adapter, Index 3 (100% over 37bp)
AGGGGCATAGTTTAAAGGTAGAACAACGAGATCGGAAGAGCACACGTCTGA308010.11450688171451386Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT303610.11287112222766649Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTGAGAGTAGGACGTCGCCACGCAGATCGGAAGAGCACACGTCTGAACT295600.10989329643456477Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGGGGTGGAGCAGTCAGGTAGCTCGTCGGGCCAGATCGGAAGAGCACAC293680.10917951047666775No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT287370.10683368266712072No Hit
TGTGAGAGTAGGACGTCGCCACGAGATCGGAAGAGCACACGTCTGAACTCC285320.10607156745165774Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGGCTTAGCTCAGTTGGTAGAGCAAGATCGGAAGAGCACACGTCTGAAC284720.1058485093398149Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGATCGTGGTGAAGTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG283770.10549533399606377Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCGATCGTGGTGAAGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA273810.10179256933947288Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGGCATAGCTCAGTTGGTAGTAGCGCATGACTGTAGATCGGAAGAGCAC273770.10177769879868336No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)