FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001839461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8985101
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC8605739.577777701107646No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT2471212.7503419271525162No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT2340292.6046340491887627Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC612570.6817619523698175TruSeq Adapter, Index 3 (100% over 50bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA474700.528319047276152Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC445610.4959432286849085Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA397950.44289986278395754Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC338000.37617829782881684No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT323850.36043000518302465No Hit
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC312290.34756426221586156Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT278970.3104806501340386Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC276330.30754245277821585Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC242810.2702362499876184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG213750.23789382000269113Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT203060.2259963466186969Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG194120.21604654193647907Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT174830.19457766807518356No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT146610.163170119067109Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA127990.14244692408020787Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT127700.14212416755248494Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG114750.12771141915933942Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC114720.12767803055302326No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT112490.1251961441501882Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC108650.1209224025417188No Hit
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA107420.11955346968275593TruSeq Adapter, Index 3 (100% over 36bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC93010.10351580911555697Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG92010.10240285557168473TruSeq Adapter, Index 3 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)