FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15973471
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC8346145.225000878018309No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC3297642.0644479837851146No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT2186551.3688634110895497No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1992221.2472054445774496Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1784981.1174653273543365No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA1748721.0947651891063626Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1366790.8556624918904601No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1080560.6764716322457404Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT1057980.6623356939765941TruSeq Adapter, Index 5 (100% over 38bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC1030110.6448880146337637No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC922910.5777767399458765No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG874440.5474326775939932TruSeq Adapter, Index 5 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT773100.4839899856455745Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT604770.3786090073973277No Hit
GGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA542210.33944406948245626Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT516040.3230606547568778Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC500950.31361374118374147TruSeq Adapter, Index 5 (100% over 50bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG430010.26920260474382807TruSeq Adapter, Index 5 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC409500.25636256515568845No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT385190.24114358112898568No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT356790.22336410164077677Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT324100.20289891908903207Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT318480.1993805854719992No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC317640.1988547135434747Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA314780.1970642448344508Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC303790.1901840871029221No Hit
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA297310.18612736079716174TruSeq Adapter, Index 5 (100% over 36bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA278520.17436410658647705TruSeq Adapter, Index 5 (100% over 40bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT262810.1645290494470488Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCGGCTGTGCGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA255080.1596897756286032Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC255050.15967099448829875No Hit
GGCGGCTGTGCGTGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC249880.15643437797583257Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC224050.1402638161737045No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT220670.1381478076994036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC179300.1122486152195725Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)