FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21045609
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT284887213.536657456669465No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC15109827.17955940357915No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC6772473.2179966851992736No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC4635472.20258297110813Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT4334972.059797841915622Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT4057101.927765549573785No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT2852191.3552423215693117No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC2613231.2416984464550302No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC1863860.8856289214534016No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1733450.8236635014933519Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC1570480.7462269207795317No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT1487420.706760255785423Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1479240.7028734592569881TruSeq Adapter, Index 6 (100% over 50bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG1325900.6300126548963254Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA1309540.6222390618394554TruSeq Adapter, Index 6 (100% over 37bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT1237040.5877900706033263Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC1204260.5722143749795978Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA1116960.5307330379463003Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT1071540.5091513388849902No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA972530.4621058958189331Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT943430.4482787834745005No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT873250.4149321599579276Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA786440.3736836505895363TruSeq Adapter, Index 6 (100% over 37bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC753570.35806519070082504TruSeq Adapter, Index 6 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC729560.3466566351204187No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT701100.3331336242158638Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA669430.31808535452692294Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT592990.28176423880154766No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG575750.273572506264846No Hit
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG553770.26312852243905127Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC505360.2401260994633132No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC479500.2278385006582608No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAAC468760.22273529837031564Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG455190.21628739752791187Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG449800.21372629321394312Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA440580.20934533184570708TruSeq Adapter, Index 6 (100% over 37bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT430810.20470303330257633Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG426770.20278339296334927Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT402020.1910232200930845Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC388250.18448028755071902No Hit
GGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC346080.1644428536137871TruSeq Adapter, Index 5 (97% over 35bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC323700.15380880638806887No Hit
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG320970.1525116236835912Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG316230.15025937239449805Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG310900.14772677759051783No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACGT309190.14691425655584497No Hit
AGGGACGGCCGGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG297560.14138816320307007Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA297450.1413358957680911Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC271850.12917183817298894TruSeq Adapter, Index 6 (100% over 36bp)
CGGAGATGGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA268150.12741375172369687TruSeq Adapter, Index 6 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC263430.1251710036045999No Hit
GGGACGGCCGGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC257420.12231530102074975TruSeq Adapter, Index 5 (97% over 35bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT252450.11995376327670063Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT243800.11584364225335556Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT228230.10844542441133445Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCTGGGTCGGTCGGGCTGGGGTAGATCGGAAGAGCACACGTCTGAACT223970.10642124920214949Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT223050.10598410338232549Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA222520.1057322693774269TruSeq Adapter, Index 6 (100% over 38bp)
CGGAGAGGGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA215720.10250119157872789TruSeq Adapter, Index 6 (100% over 37bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC212670.10105195815431144TruSeq Adapter, Index 6 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)