FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20460305
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC422615720.655395899523494No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT7931023.876296076720264No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT3753651.8346011948502234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA2858461.3970759477925672Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC2015960.9853030050138548No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1525260.7454727581040458Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1275200.6232556161797197No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC924060.451635496147296Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC809060.39542910039708595No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT782360.3823794415576894Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC682900.33376824050276865TruSeq Adapter, Index 10 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT615920.30103168061277674Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC520560.2544243597541679Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC453550.22167313732615423TruSeq Adapter, Index 10 (100% over 37bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC431180.21073977147456993TruSeq Adapter, Index 10 (100% over 37bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG402310.19662952238493023Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG395200.19315450087376507Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG332170.1623485084899761TruSeq Adapter, Index 7 (97% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT328230.16042282849644712No Hit
GCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT300440.14684043077559206Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT298220.1457554029619793TruSeq Adapter, Index 10 (100% over 38bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT296140.1447388003258016Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTCT286330.139944150392675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT282540.13809178308925502TruSeq Adapter, Index 10 (100% over 39bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC264920.12947998575778805TruSeq Adapter, Index 10 (100% over 37bp)
GCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC258870.12652304059005964Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT254820.12454359795711745Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT254050.12416725948122476TruSeq Adapter, Index 10 (100% over 38bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT249010.12170395309356336Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA230820.11281356753968233Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC230730.11276957992561694No Hit
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT222890.1089377699892548Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT214340.10475894665304354TruSeq Adapter, Index 10 (100% over 39bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT208300.10180688899798904No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)