FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15557296
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC6194963.982028753582885No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT4347892.7947594491999124No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2104391.3526707983186794Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG1366130.8781281785729345Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG1258400.8088809263512118TruSeq Adapter, Index 5 (100% over 37bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG1200970.7719657709154598Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA1101070.707751527000579TruSeq Adapter, Index 5 (100% over 36bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC909770.5847867135779894Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA895600.5756784469486214TruSeq Adapter, Index 5 (100% over 36bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA871100.5599302089514785Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC754520.4849943074940529Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT724500.46569789505837Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT707270.45462270564242013No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC656050.42169924645002577TruSeq Adapter, Index 5 (100% over 50bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA591140.37997605753596253Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG584730.37585580424773046Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC574400.3692158328799555Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA558990.3593105125723648TruSeq Adapter, Index 5 (100% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT521890.3354631807481197No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG519490.3339204962096241TruSeq Adapter, Index 5 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC501460.32233107861417565No Hit
AGGAAACTCTGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC481660.30960393117158663TruSeq Adapter, Index 5 (100% over 35bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG472410.3036581678461347TruSeq Adapter, Index 5 (100% over 37bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT439340.2824012604761136Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG383770.24668168555769587No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT378200.2431013718579373Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA357960.23009139891662406Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATGGATTTTTGGAGCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT350990.22561118590274298Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG344000.22111811718437446No Hit
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG342450.22012180008659601Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC314090.20189241112337258No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT304450.19569596156041513Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT287790.18498715972235794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA286370.184074404703748Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT279120.17941421182704242TruSeq Adapter, Index 5 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC277720.17851431251292No Hit
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG273910.17606530080805816TruSeq Adapter, Index 5 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT269040.17293493676536076No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC253580.1629974771965514No Hit
CGGAGGAGAAGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG245020.15749523567591694TruSeq Adapter, Index 5 (100% over 37bp)
AGGGACGGCCGGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA238280.1531628632636417Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGCGGGGAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC227280.1460922257955367TruSeq Adapter, Index 5 (100% over 35bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACAGATCGGAAGAGCACA201910.12978476465318908No Hit
GGGACGGCCGGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC199550.12826779152366838TruSeq Adapter, Index 5 (100% over 35bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA198760.12775999119641357Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGTCTGAACTCCAG196460.12628158518035523Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT195320.12554881002456983TruSeq Adapter, Index 5 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT195220.1254845315021325No Hit
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA191130.12285553993444619TruSeq Adapter, Index 5 (100% over 36bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT187010.12020726481002868No Hit
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA177610.11416508370092077Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGTGTGACAATGGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCAG176230.1132780400912858Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA174720.1123074344024823TruSeq Adapter, Index 5 (100% over 36bp)
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG173320.11140753508835982TruSeq Adapter, Index 5 (100% over 39bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC167760.10783364924084493No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA166930.1073001375046152Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGCTGAGAAGACGGTCGAACAGATCGGAAGAGCACACGTCTGAACTCCA166510.10703016771037846Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC164280.10559675666002627TruSeq Adapter, Index 5 (100% over 35bp)
GGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG161230.10363626172568807TruSeq Adapter, Index 5 (100% over 37bp)
GAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC160980.10347556541959477TruSeq Adapter, Index 5 (100% over 35bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG156910.10085942955639592Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)