FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16574234
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG9907205.977470814035811Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT8822075.322761824166354No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC7980884.815233090108418No Hit
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC3460502.0878792950552043TruSeq Adapter, Index 6 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT2266471.367465911245129Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2128771.2843851486590572Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1551630.9361699611577826No Hit
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA1524620.9198735820913353TruSeq Adapter, Index 6 (100% over 37bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1456300.8786529742490664Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1093370.6596805620096833TruSeq Adapter, Index 6 (100% over 50bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT930560.5614497780108572Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA828060.4996067993247833Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA658130.3970801908552757TruSeq Adapter, Index 6 (100% over 37bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA654850.39510121553732136TruSeq Adapter, Index 6 (100% over 37bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG614880.3709854705804202Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA609620.36781186991809095TruSeq Adapter, Index 6 (100% over 38bp)
ACGGCCCTGGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC597690.36061395054516543TruSeq Adapter, Index 5 (97% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT517980.31252123024207334Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC452750.2731649619523895No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT412760.24903715007281785No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC398710.2405601368968243No Hit
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA380500.22957320380537646TruSeq Adapter, Index 6 (100% over 37bp)
GGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA345520.20846815605475344TruSeq Adapter, Index 6 (100% over 37bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA314320.1896437566888461TruSeq Adapter, Index 6 (100% over 38bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG287490.1734559799264328Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT286880.17308793878498396No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT269750.16275261951774062No Hit
GAGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC266800.16097274842384873TruSeq Adapter, Index 5 (97% over 35bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC258680.15607357781964465TruSeq Adapter, Index 6 (100% over 36bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC257300.15524096015538336No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC251570.15178378681029844Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC245830.14832058000387832TruSeq Adapter, Index 6 (100% over 36bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC244160.14731299196089545No Hit
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA236650.14278186249814018TruSeq Adapter, Index 6 (100% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC230950.13934278953706095No Hit
ACGGCCCTGGCGGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC215560.13005729254214704Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACGGGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT213490.12880836604575513TruSeq Adapter, Index 6 (100% over 39bp)
ACGGCCCTGGCGGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA212180.1280179826108404Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA211330.12750513839734615TruSeq Adapter, Index 6 (100% over 37bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC210170.12680525688245986TruSeq Adapter, Index 6 (100% over 36bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG205020.12369802429481808Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG198960.1200417467256707Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA194030.11706725028740393TruSeq Adapter, Index 6 (100% over 38bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT193010.1164518372312108Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)