FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15085831
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC4299872.8502705618271875No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT3670572.4331241679692686No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC3153382.0902925400662387Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC1874701.2426892492697286Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1806041.1971763438155976TruSeq Adapter, Index 7 (100% over 50bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG1231750.8164946299610543TruSeq Adapter, Index 7 (100% over 36bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1170870.7761388815770242Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1110150.7358891929784975TruSeq Adapter, Index 7 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT621050.41167768616790157No Hit
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG611080.40506883578372316TruSeq Adapter, Index 7 (100% over 36bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG569940.37779821343617065Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA554120.36731155214452554TruSeq Adapter, Index 7 (100% over 37bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA519940.3446545304663694Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA501540.3324576551334826Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC455560.3019787242744533Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC421980.27971942679193473Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT394220.2613180540071011Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA390940.25914382840428213TruSeq Adapter, Index 7 (100% over 37bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC362750.24045741994590816Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT327180.21687900388119158Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT320280.21230517563135898Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC320130.2122057445824496Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG300780.19937913927313647TruSeq Adapter, Index 7 (100% over 36bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC282110.1870032880522127Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG278210.1844180807805682Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT259110.1717571938861041No Hit
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC249530.1654068642290902Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG247690.16418717669580152Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT232160.15389274876538123No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC229450.152096361148418No Hit
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG224810.14902062736882044Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA208430.13816275682791357Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCCCTGGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA197590.13097720636006063TruSeq Adapter, Index 7 (100% over 35bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC196250.13008895565646997TruSeq Adapter, Index 7 (100% over 39bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT194860.1291675612699095TruSeq Adapter, Index 7 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC194250.1287632083376779No Hit
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT192970.127914730053651TruSeq Adapter, Index 7 (100% over 38bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC192860.12784181395111743Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA189630.12570073203126828TruSeq Adapter, Index 7 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG182750.12114016125462364No Hit
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT180010.11932388742787851Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT179730.11913828280324763TruSeq Adapter, Index 7 (100% over 38bp)
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC176490.1169905721468045TruSeq Adapter, Index 7 (100% over 39bp)
AGCGGGGCTGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG172870.11459096949979089TruSeq Adapter, Index 7 (100% over 36bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAAC172480.11433244877262645Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA172430.11429930508965663Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA169770.11253606115566321Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC169700.1124896599995055No Hit
AACGGGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC156940.10403139210561221TruSeq Adapter, Index 7 (100% over 39bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC156930.10402476336901825No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT155660.1031829138215853Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAAACTCTGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA155100.10281170457232354TruSeq Adapter, Index 7 (100% over 35bp)
GAGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA154170.10219523206908522TruSeq Adapter, Index 7 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)