FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001839489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9783508
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC7747627.919061342823045No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC2172352.220420323671223TruSeq Adapter, Index 11 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT1007341.0296306805289064No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT928850.9494038334715932Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC616910.6305611443257367Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC509000.5202632838854938Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC453550.46358627191800733Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG340640.3481777701822291Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA320190.3272752472834897Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC311940.3188426891458565No Hit
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT282900.2891600845013874Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT273820.27987915990869533Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC236570.24180488225695732No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC201340.2057953036886156Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA175880.17977191821174981No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG155550.15899205070410327Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG153660.15706022829439092Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG136640.13966360532438876No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT134140.1371082846766211No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA121610.12430101759000962Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTGAGATCGGAAGAGCACACGTCTGAACTCCA118730.1213572882037813Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT116010.11857709933901009Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAGATCGGAAGAGCACACGTCTG113120.11562314867019069Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT98600.10078184634795617Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)