FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20784980
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC11055055.318768649284243No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT9300524.474635048963242No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT3026801.456243883804555Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC2141581.030349800673371Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC1864260.8969265306004625Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1800810.8663996789989694TruSeq Adapter, Index 6 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1793750.8630029954322784No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC1747040.8405300365937327No Hit
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA1650100.7938905882998204Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1468370.7064572590399413Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1233480.593447768532854No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT653820.3145636897413421No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT604120.2906521921118038Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC590090.28390212547714744No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC541760.26064975766154214No Hit
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT531630.2557760459716584Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC497750.23947581378476185No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT497690.23944694678561151No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA473120.227625910633544Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAAATAGGAGAGATCGGAAGAGCACACGTCTGAACTCC455030.21892251038971414Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA438950.21118615461742082Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAAATAGGAGAAGATCGGAAGAGCACACGTCTGAACTC410720.19760423151718212Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT375060.18044761168882528No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG372020.17898501706520767Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC356280.17141224095476637No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT331340.15941319164127174Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT297650.14320437161835134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAAC288100.13860970758692093Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATGGATTTTTGGAGCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT280800.1350975560236286Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT277740.13362533906696086Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG276390.132975831586078Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTAGATCGGAAGAGCACACGTCTGAACTCC265780.1278711839029915Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG253620.12202080540852096No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC238210.1146067977934066Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA224560.10803955548670241No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT223820.10768352916384813Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA218200.10497965357676553Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)